Antibacterial Activity of Ethanolic Root Extract of Fibraurea tinctoria Lour. Against Diabetic Wound Pathogenic Bacteria

Abstract

This study examined the in vitro antibacterial activity of ethanolic root extract from Fibraurea tinctoria Lour. against two clinically relevant diabetic wound pathogens, Staphylococcus aureus and Pseudomonas aeruginosa. Using a Completely Randomized Design, the extract was tested at concentrations of 2%, 4%, 6%, 8%, and 10% via the disk diffusion method, and the resulting data were analyzed with one-way ANOVA followed by Duncan's Multiple Range Test. A concentration-dependent inhibition of S. aureus growth was observed (p < 0.001), reaching its peak at 10% (13.67 ± 0.82 mm), while P. aeruginosa showed no measurable susceptibility at any concentration tested. Taken together, these results point to a selective antibacterial effect of F. tinctoria root extract against Gram-positive bacteria, positioning it as a promising candidate for further exploration as a natural antibacterial agent in the management of diabetic wound infections.