2026· Methods in molecular biology· Vol 3047, pp.
225-235
· 0 citations
Medicine
TL;DR
A metabolic labeling approach with 5-ethynyl uridine (5-EU) that enables tracing of EV-mediated RNA transfer under physiological conditions and preserves native RNA loading into EVs and enables tracing their delivery to recipient cells, providing a reliable and minimally perturbative approach for studying EV-mediated RNA transport in intercellular communication.
Extracellular vesicles (EVs) are fundamental mediators of intercellular communication that transfer macromolecular cargo, including proteins and RNAs, to functionally reprogram recipient cells. However, the molecular processes by which transferred ribonucleoprotein (RNP) complexes engage recipient host machinery to inf...
Rajat Dhyani, Stephen Clarke, Masashi Kuroda et al.· bioRxiv· 0 citations
: Extracellular vesicles (EVs) can transport functional RNA, but the clinical development of EV-based RNA therapeutics has been constrained by the technical demands of ex vivo production and cargo loading. A recently reported strategy uses a plasmid to direct the formation of small interfering RNA (siRNA)-containing sm...
Extracellular vesicles (EVs), particles released from cells, have the potential to become an important in vivo delivery vehicle for CRISPR machinery. Unlike viral vectors such as Adeno-associated virus (AAV), EVs reduce risks associated with immunogenicity, long-term expression, and potential off-target effects. EVs ca...
Peter Pečan, Mateja Manček-Keber· Methods in molecular biology· 0 citations
Plant extracellular vesicles (EVs) have emerged as candidate carriers of small RNAs that connect intracellular RNA regulation with apoplastic defense, intercellular signaling, and cross-kingdom communication. The field is advancing rapidly, yet its central mechanistic claims remain unevenly resolved because RNA detecti...
Aziz Ullah· International Journal of Bio...· 0 citations
Extracellular vesicles (EVs) are membrane-bound, nano-sized particles released by diverse cell types. They serve as key mediators of intercellular communication by transporting a broad repertoire of proteins, lipids, nucleic acids, and metabolites to recipient cells within a protective lipid bilayer. Owing to their nat...
Nawaz Khan, Zeynab Ahmadova, Yu-Jie Shi et al.· Molecular Biomedicine· 1 citation
Outer membrane vesicles (OMVs), as promising delivery vectors, have been extensively explored for the delivery of nucleic acid-based drugs, particularly the RNA therapy. However, RNA-loaded OMVs delivery systems derived from in vivo engineered bacteria lack the controllable way for regulating target RNA synthesis. Co...
