Next-generation experimental techniques for mRNA lipid nanoparticle evaluation and protein interaction analysis

Abstract

The promise of RNA therapeutics is tanta-lizing to drug delivery scientists, as RNA-en-coded genes generally need only reach the cytoplasm to perform their therapeutic functions, circumventing the need for nuclear localization to achieve transcription. 1 However, targeted intracellular delivery of mRNA is difficult, requiring precise tuning of carriers to transfect target tissues and cells. The premier mRNA delivery technology is lipid nanoparticles (LNPs), which possess adjuvant properties that make them particularly appealing for mRNA vaccines. 1 mRNA LNPs have demonstrated promise extending well beyond vaccine applications, with studies evaluating their use to treat cancer, autoimmunity, pregnancy disorders, and beyond. 1–4 The