Sep 2026· Journal of Agricultural and Food Chemistry· Vol 74, pp. 32502-32513· 0 citations· 38 references
Medicine
TL;DR
This work establishes a green and sustainable platform for producing active ginsenoside aglycone analogues, paving the way for the development of drugs and functional foods.
Abstract
The pharmacological activity of protopanaxadiol (PPD) can be enhanced by oxidative modification. Compared with chemical synthesis and biotransformation, synthetic biology offers a more efficient and eco-friendly approach for modifying PPD. In this study, we identified two oxidases from Mucor spinosus. MsSDR3 oxidizes C3-OH of both dammarenediol-II and PPD to a ketone, while MsCYP3 oxidizes C12-OH of PPD to a ketone and hydroxylates PPD at C7β, C15α, and C11β positions. Combining MsSDR3/MsCYP3 with the enzymes involved in ginsenoside biosynthesis, we achieved de novo biosynthesis of seven ginsenoside aglycone analogues in Saccharomyces cerevisiae. Pharmacological evaluation indicated that 12-oxo-15α-hydroxy-protopanaxadiol (p3) showed higher anticolon cancer activity, and 7β-hydroxy-protopanaxadiol (p6) exhibited not only higher anticolon, antigastric, antiliver, antilung, and antipancreatic cancer activities but also higher cardioprotective activity than PPD. Our work establishes a green and sustainable platform for producing active ginsenoside aglycone analogues, paving the way for the development of drugs and functional foods.
Protopanaxadiol (PPD)-type ginsenosides, as the primary bioactive components of Panax medicinal plants, have become a research hotspot in natural products due to their diverse pharmacological activities. The biosynthetic pathway of PPD-type ginsenosides in Panax species and the related enzymes is becoming increasingly...
Na Li, Xing-Yin Jiang, Shi-Song Gao et al.· ACS Omega· 0 citations
Acoranes represent an important family of sesquiterpenes featuring a unique spiro[4.5]decane skeleton with remarkable structural diversity and extensive biological activities. In this study, genome mining of the plant pathogenic fungus Rhynchosporium agropyri enabled the identification of a small biosynthetic gene clus...
Hai-Chun Zeng, Bei-Lin Meng, Yu-Xin Zhou et al.· Journal of Agricultural and...· 0 citations
Endophytic fungi are significant sources of structurally diverse natural products with promising pharmacological and biological applications. In this study, endophytic fungi isolated from Ocotea diospyrifolia leaves were investigated as potential producers of anti-inflammatory natural products using an integrated workf...
G. S. Oliveira, M. Marques, Ana L. U. Nascimento et al.· Molecules· 0 citations
Marine brown algae are an important source of bioactive metabolites with promising anticancer potential. Scytosiphon lomentaria has repeatedly shown selective antitumor activity, although the active metabolite associated with its lipophilic fractions had not previously been identified. Here, five solvent extracts were...
Matteo Mauceri, Abdul-salam Juhmani, R. Zangrando et al.· Marine Drugs· 0 citations
Coumarins are heterocyclic compounds with diverse biological activities and potential applications as alternative acaricidal agents. In this study, nine coumarin derivatives were synthesized through a solvent-free Pechmann reaction using Preyssler heteropolyacid as a reusable catalyst under green chemistry conditions....
Carlos Rodríguez, Diego M. Ruiz, Alejandra Rodríguez et al.· Molecules· 0 citations
Abstract Paeoniflorin is a pharmacologically important cage-like monoterpene glycoside characteristic of Paeonia plants, yet its biosynthetic pathway has remained largely unresolved, hindering sustainable production. Here, we confirmed that paeoniflorin biosynthesis originates from α-pinene and identified 3 novel cytoc...
Xi-Rui Tan, Xin-Ran Chen, Long-Ji Wang et al.· Plant Physiology· 0 citations
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