Aug 2026· Jurnal Fitopatologi Indonesia· Vol 22, pp. 40-47· 0 citations· 23 references
Abstract
Moler disease caused by Fusarium acutatum represents a major constraint in shallot production. The use of biological control agents has emerged as an environmental friendly disease management. This study aimed to evaluate the effectiveness of a combined application of Trichoderma sp. and Bacillus velezensis B-27 in suppressing moler disease and to assess its effects on plant growth and yield of shallots. The study consisted of in vitro antagonistic assays and in vivo glasshouse experiments using two shallot varieties derived from botanical seeds or true shallot seed (TSS). The observed parameters included pathogen growth inhibition, disease intensity, number of bulbs, bulb diameter, fresh bulb weight, and dry bulb weight. The results demonstrated that the combined biological agents effectively inhibited pathogen growth and significantly reduced moler disease intensity. Although several yield-related parameters did not differ significantly among treatments, the application of biological agents showed considerable potential as an eco-friendly and sustainable approach for managing moler disease in shallot cultivation.
The increasing difficulty of controlling watermelon Fusarium wilt disease (caused by Fusarium oxysporum f. sp. niveum, Fon) in continuous-cropping systems using traditional chemical control techniques highlights the need for sustainable alternative strategies. This study evaluated the biocontrol agent Trichoderma koningiopsis T-51 through controlled assays and multi-year field trials between 2020 and 2024 at multiple sites in Shanghai, China. T-51 exhibited the most effective activity in susceptible genotypes, reducing the disease index by 76.5% in the susceptible watermelon line W4k-1 in controlled assays. Field trials revealed dose-dependent effects, with treatment using T-51 at high concentrations (5×10⁷ CFU/mL, repeated drenching) reducing disease incidence by 59 to 76% in continuous-cropping fields. In contrast, low concentrations (5×10⁶ CFU/mL) advanced flowering by 6 days and increased average fruit number per plant by 15 to 20%. Marketable fruit yields increased by 15 to 18% in T-51-treated plots without compromising fruit quality during continuous cropping in large fields. T-51 stably colonized soil and reshaped soil microbiomes, suppressing Fusarium populations, and enriching potentially beneficial bacteria (Bacillus and Pseudomonas) in continuous-cropping fields. The broad fungicide compatibility of T-51 treatment indicates that it could be used in combination with fungicides. Overall, T-51 proves to be an effective and sustainable tool for integrated management of watermelon Fusarium wilt, particularly for high-yield, disease-susceptible cultivars, and offers a sustainable alternative to chemically intensive practices.
Jiaqi You, Lihua Zhu, Zheng Hu et al.· Plant Disease· 0 citations
The dependence on synthetic fungicides in smallholder vegetable production creates both an economic and environmental concerns in sub-Saharan Africa. This study evaluated the biocontrol potential of two molecularly identified Trichoderma isolates, T. viride and T. harzianum, against Fusarium oxysporum and Cucumber mosaic virus (CMV) in tomato under controlled screenhouse conditions. The isolates were characterized using morphological traits and confirmed by ITS and tef1 sequencing. In vitro antagonism against F. oxysporum was assessed using serial spore concentrations, while in vivo efficacy was evaluated through pathogen challenge experiments measuring disease incidence, severity, plant growth, chlorophyll content, biomass, and root colonization over 12 weeks. Both isolates significantly suppressed pathogen activity, with T. viride showing stronger overall performance. In vitro, T. viride achieved 94.3% inhibition of F. oxysporum at 10⁹ CFU mL⁻¹ after 120 hours, compared with 91.7% for T. harzianum at same end point. Under screenhouse conditions, T. viride reduced Fusarium wilt incidence by 67% and CMV severity by 67%, whereas T. harzianum reduced Fusarium wilt incidence by 60% and CMV severity by 59% relative to pathogen-inoculated controls. Plants treated with T. viride also exhibited the greatest plant height, chlorophyll content, biomass accumulation, and root colonization, indicating enhanced plant vigour. In contrast, carbendazim effectively suppressed Fusarium wilt but provided no protection against CMV. These findings demonstrate that both Trichoderma species function as multifunctional bioagents capable of simultaneously suppressing fungal and viral diseases while promoting tomato growth, with T. viride showing superior overall efficacy and strong potential for sustainable integrated disease management.
E. E. Ekpiken· FUDMA Journal of Agriculture...· 0 citations
The results show that the formulated consortium offers an innovative, stable, high quality, and environmentally friendly substitute to chemical fungicides for use in Integrated Disease Management (IDM) programs.
Thulfiqear A. Jabbar, A. al-abedy, Abdulzahra J. Ali· Brazilian Journal of Microbi...· 0 citations
The antifungal effects of some botanical extracts and the antagonistic effects of Trichoderma asperellum isolates against Alternaria brassicicola, the causative agent of Alternaria blight of mustard was evaluated in this study on November 2021 to February 2022 at Bangladesh Agricultural University, Mymensingh. A total of 18 plant extracts and 18 Trichoderma isolates were used to evaluate their effectiveness in inhibiting A. brassicicola mycelial growth. The highest mycelial growth inhibition (93.59 %) was observed with cumin seed and clove seed extracts. Five T. asperellum isolates exhibited 84.13 % growth inhibition of A. brassicicola in in vitro dual culture assays. Following these promising findings, field trials were conducted using the best two botanicals at two different concentrations (1:10 and 1:20) and formulated products of two T. asperellum isolates (BDPMIL-TAN51 and BDPMIL-TAN41) to evaluate their impact on disease incidence and severity of Alternaria blight in the field condition. In field conditions, plants treated with T. asperellum bioformulation demonstrated superior results compared to those treated with botanicals, exhibiting minimal disease incidence and severity (19 % and 18.33 % respectively), while water control plants recorded the highest disease incidence and severity (38 % and 78.33 %) at 54 days after sowing (DAS). Trichoderma asperellum isolate PMIL-TAN51 bioformulation-treated plants displayed the lowest pod infection rate (20 %), whereas control plants presented the highest pod infection rate (66.63 %). Additionally, plots sprayed with Trichoderma bioformulation-treated plants showed better yield-contributing parameters and overall yield compared to those treated with botanicals. According to the results of field evaluation, T. asperellum, as a biocontrol agent, may help in managing Alternaria blight of mustard disease in Bangladesh.
A. Muhammed, S. Nadira, T. Fatema et al.· Plant Science Today· 0 citations
Fusarium wilt disease caused by Fusarium oxysporum f. sp. melonis is a major factor reduces of melon (Cucumis melo L.) production in Indonesia. Control using chemical fungicides has not been effective and poses risks to the environment and health. The aim of this experiment was to find out more accurate treatment combination of Trichoderma spp. as biological control agents with neem leaf powder (Azadirachta indica) as a botanical fungicide for inhibiting the growth of F. oxysporum in vitro. The Trichoderma species used in this study were collected by the Plant Pathology Laboratory collection of Udayana University. The research was conducted using a Randomized Complete Design (RCD) with two factorial treatments comprising three Trichoderma species (T. harzianum, T. koningii, and T. viride) and four neem leaf powder concentrations (0 g L⁻¹, 15 g L⁻¹, 30 g L⁻¹, and 45 g L⁻¹) and 3 replications. Antagonistic activity was assessed using the dual culture method on potato dextrose agar (PDA) medium, with observations conducted over seven days observed parameters included pathogen colony growth and percentage of inhibition. The results showed that the combination of Trichoderma spp. and neem leaf powder significantly inhibited the growth of F. oxysporum in vitro, with varying effectiveness among Trichoderma species. The best inhibition was obtained from the combination of Trichoderma koningii and neem leaf powder at a concentration of 45 g L⁻¹, which completely inhibited the growth of F. oxysporum (100%) by day 5 without suppressing the growth of Trichoderma.
R. Pakpahan, I. K. Suada, T. A. Phabiola· International journal of mul...· 0 citations
Scanning electron micrographs of Sclerotium rolfsii paired with effective Trichoderma and Bacillus isolates showed swollen hyphae with cell-wall damage, providing clear evidence of antagonistic interactions caused by volatile compounds produced by B. amyloliquefaciens and T. harzianum.
A. A. Khan, V. Bhuvaneswari, P. Anisha et al.· Asian Research Journal of Ag...· 0 citations
We use cookies to run the site and, with your consent, for analytics and to show ads.
See our Cookie Policy.
