Jul 2026· Journal of Agricultural and Food Chemistry· 0 citations· 31 references
Medicine
TL;DR
Findings indicate that FaLEC2 modulates PCs and LA by regulating lipid-related genes, along with a set of candidate TFs, thereby contributing to the regulation of phospholipid and linoleic acid metabolism in strawberries.
Abstract
Lipids are vital cellular components with structural, storage, signaling, and defensive functions, and lipid metabolism is critical to strawberry quality. However, the regulatory mechanism underlying lipid metabolism during strawberry development and ripening remains unclear. Although LEAFY COTYLEDON 2 (LEC2) regulates lipid metabolism, its function in strawberries is unknown. This study explored the role of FaLEC2 in strawberries. FaLEC2 is highly expressed during strawberry development. FaLEC2 overexpression delayed ripening, promoted the accumulation of phosphatidylcholines (PCs), including 1,2-dipalmitoyl-3-sn-phosphatidylcholine (DPPC) and PC (16:0/18:3(9Z,12Z,15Z)), and linoleic acid (LA), upregulated FaLOX2.4/2.5/3, downregulated FaLOX5-like2, and regulated various transcription factors (TFs). Correlation analysis revealed associations among lipid metabolites and multiple regulatory genes. Yeast one-hybrid and dual-luciferase reporter assays preliminarily suggested a regulatory association between FaLEC2 and FaPLA1, identifying FaPLA1 as a putative direct target. These findings indicate that FaLEC2 modulates PCs and LA by regulating lipid-related genes, along with a set of candidate TFs, thereby contributing to the regulation of phospholipid and linoleic acid metabolism in strawberries.
The nesfatin-1 gene could influence the early development stage of largemouth bass by affecting signaling pathways associated with glycolipid metabolism, and provide further theoretical support for the initial breeding and feed adaptation of largemouth bass.
Shiping Su, Xin-Xin Liu, Junqing Li et al.· Genomics· 0 citations
Key genes in the mevalonate (MVA) pathway were upregulated in roots, facilitating metabolic crosstalk with the methylerythritol-phosphate pathway and enhancing terpenoid skeleton synthesis.
Results provide a genetic entry point for metabolic engineering and breeding efforts aimed at modulating grain phenolic profiles and indicate that SbmiR397-5p negatively regulates the expression of SbLAC14 in relation to CT biosynthesis, identifying it as a potential target for manipulating CT metabolism in sorghum.
Yannan Shi, Yongchao Guo, Jinping Wang et al.· Plants· 0 citations
Sesame is an important oilseed crop, and floral development is a key biological process that lays the foundation for pollination, fertilization, and seed formation, which are closely associated with final yield potential. However, the dynamic transcriptional and metabolic regulatory mechanisms during floral development remain unclear. Here, we performed an integrated transcriptomic and metabolomic analysis across five key developmental stages (T1–T5) of sesame flowers to systematically dissect the multi‑omics regulatory network. KEGG enrichment analysis revealed distinct stage‑specific metabolic characteristics: early stages (T1–T2) were enriched in primary energy metabolism (glycolysis and starch/sucrose metabolism); the middle stage (T3) showed enrichment in DNA replication and phenylpropanoid biosynthesis; and late stages (T4–T5) were associated with plant hormone signaling and α‑linolenic acid metabolism. WGCNA identified two modules correlated with development: a positive module involved in phenylpropanoid biosynthesis, and a negative module related to DNA replication and repair. Genes in the phenylpropanoid/flavonoid pathway displayed a clear sequential expression pattern, promoting flavonoid and anthocyanin accumulation. Collectively, this study provides a comprehensive multi‑omics resource and a descriptive framework for understanding transcriptional and metabolic dynamics during sesame floral development, and identifies candidate pathways and genes that may serve as targets for future functional validation and molecular breeding.
Qiyuan An, Hongsen Cheng, Huijie Sun et al.· Frontiers in Plant Science· 0 citations
: Mint is notably rich in phenolic acids, flavonoids, antioxidants and other bioactive components, and is widely used as food, medicine, spices, and flavoring agents. Thus, metabolite composition serves as a critical indicator for assessing mint quality. In this study, two mint genotypes of Mentha canadensis L., were sampled, namely purple mint and green mint. The two genotypes are distinguished by stem color: the purple mint exhibits purple stems, whereas the green mint has green stems. The purple mint exhibited significantly higher anthocyanin and total flavone contents than green mint. Integrated transcriptomic and metabolomic analyses were performed to elucidate the regulatory mechanisms underlying pigment and flavonoid accumulation in mint stems. High-throughput RNA-Seq yielded 167,901 unigenes, of which 34,608 genes were differentially expressed. These differentially expressed genes (DEGs) were mainly involved in the lignin metabolic process and flavonoid biosynthetic process. A total of 143 differentially expressed metabolites (DEMs) were enriched in isoflavonoid, flavonoid biosynthesis, flavone and flavonol biosynthesis, and anthocyanin biosynthesis pathways. Co-analysis of DEGs and DEMs revealed that the flavone and flavonol biosynthesis pathway (ko00944) contained the most DEMs, followed by the flavonoid biosynthesis pathway (ko00941) and the anthocyanin biosynthesis pathway (ko00942). Furthermore, nine key genes and metabolites were identified using the O2PLS model. These findings provide a theoretical basis for understanding the key pathways and genes involved in pigment and flavonoid regulation in mint stems.
Xiang-Dong Wang, Hai-Long An, Yan-Zhi Ma et al.· Phyton· 0 citations
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