Findings demonstrate that CtDnaJ.16 plays a strikingly haploid-specific role in thermotolerance, deepening the understanding of functional differentiation between allelic genes.
Abstract
The two haploids, Ct001_29 and Ct001_31, derived from the thermotolerant strain Coriolopsis trogii Ct001, differ in mating type and exhibit distinct thermotolerance capacities. To investigate the underlying molecular mechanisms, their phenotypic responses to different temperatures were characterized, and comparative RNA-sequencing (RNA-Seq) analyses were performed using an upgraded, manually curated genome annotation. Phenotypic observations revealed that Ct001_31 displayed more robust hyphal growth at 35 °C than Ct001_29, including denser mycelia, larger hyphal diameter, smoother hyphal surfaces, and the ability to sustain growth at 40 °C. Through manual correction, a high-quality genome annotation for Ct001_31 was generated, comprising 12,953 high-confidence genes. Comparative RNA-Seq analysis identified 108 up-regulated and 121 down-regulated genes commonly shared between the two haploids at 35 °C. The up-regulated genes in Ct001_29 were significantly enriched in functions related to protein folding and heat response, whereas no significant functional enrichment was observed among the differentially expressed genes (DEGs) in Ct001_31 at 35 °C. Notably, CtDnaJ.16 showed increased expression in Ct001_31 at 35 °C, while no significant change was observed in Ct001_29. Functional investigation revealed that Ct31DnaJ.16 was specifically involved in thermotolerance in Ct001_31, with transformants overexpressing this gene capable of germinating at 43 °C. In contrast, neither overexpression nor RNA interference of Ct29DnaJ.16 in Ct001_29 led to detectable phenotypic alterations. These findings demonstrate that CtDnaJ.16 plays a strikingly haploid-specific role in thermotolerance, deepening our understanding of functional differentiation between allelic genes.
An association-based multi-module framework of candidate traits linked to Calditerricola persistence during HC is defined, providing a genome-resolved basis for prioritizing testable thermotolerance targets in engineered high-temperature waste-treatment systems.
Xu Li, Zhengnan Zhu, Youzhao Wang et al.· Bioresource Technology· 0 citations
Sucrose non-fermenting-1-related protein kinase (SnRK) is a plant serine/threonine kinase that mediates stress signaling, yet its function in the mangrove Avicennia marina remains unexplored. In this study, we identified 46 SnRK genes in A. marina, classifying them into three subfamilies with high phylogenetic conservation. Family expansion occurred mainly through 20 segmental duplication events under purifying selection (Ka/Ks < 1). Promoter regions were enriched in stress- and hormone-responsive elements, and expression profiling showed distinct tissue-specific and stress-responsive patterns. Our study specifically focused on the functional characterization of AmSnRK2.7. AmSnRK2.7-overexpressed Arabidopsis thaliana increases salt tolerance by altering the expression of ion transport genes and mediating Na+ efflux from the roots. Further investigation revealed that AmSnRK2.7 interacts with and phosphorylates AmENO2 (enolase, a key enzyme in the glycolysis pathway). Meanwhile, measurements of ATP content in wild type, AmSnRK2.7-overexpressed Arabidopsis lines and the atsnrk2.6 (homologous gene of AmSnRK2.7 in A. thaliana) mutant confirm that the AmSnRK2.7-AmENO2 module can affect the energy-driven Na+ efflux. Our findings provide key insights into the role of SnRK gene family in mangrove adaptation to saline intertidal habitat, and suggest AmSnRK2.7-AmENO2 module plays a role in salt tolerance.
Jin-Yu Liu, Yudan Xiang, Lingyu Song et al.· Plant, Cell and Environment· 0 citations
The BASIC PENTACYSTEINE (BPC) family represents a group of plant-specific transcription factors with established functions in developmental regulation and environmental adaptation. Although BPC genes have been catalogued in diverse plant species, their genome-wide characterization and light-dependent expression dynamics remain unexplored in the medicinal herb Peucedanum praeruptorum Dunn. Here, we report the systematic identification of eight PpBPC loci from the P. praeruptorum genome assembly through comprehensive bioinformatic screening. Evolutionary reconstruction grouped these eight members into three distinct clades (designated A, B, and C). Comparative genomic analyses revealed pronounced syntenic conservation between PpBPCs and their counterparts in Arabidopsis thaliana, Angelica sinensis, and Daucus carota. Members clustered within the same phylogenetic group displayed analogous exon-intron architectures and conserved motif repertoires, and all possessed the characteristic GAGA-binding domain. Examination of transcript abundance across organs demonstrated that clade A and C members shared broadly overlapping expression signatures in root, stem, and leaf tissues, whereas clade B genes exhibited organ-prevalent patterns. When seedlings were treated with three monochromatic light regimes, the PpBPC family displayed heterogeneous transcriptional responses, with individual clades showing distinct wavelength sensitivities. In particular, PpBPC6 and PpBPC8 were strongly activated by blue light. Collectively, these data delineate the foundational landscape of the PpBPC family and highlight candidate members likely involved in light signal transduction, thereby establishing a framework for future mechanistic dissection of light-responsive gene regulation in P. praeruptorum.
Findings substantiate a positive regulatory role of Mn-Tret1 in governing growth performance in M. nipponense and contributes to a deeper mechanistic understanding of growth regulation in this freshwater prawn species.
Shubo Jin, Zijian Gao, Y. Xiong et al.· International Journal of Mol...· 0 citations
Wheat stripe rust (yellow rust), a devastating disease caused by the phytopathogen Puccinia striiformis f. sp. tritici (Pst), poses a major threat to global wheat (Triticum aestivum L.) production. Under ongoing climate warming, the highly virulent Pst race CYR34 has become predominant in China and exhibits increased tolerance to elevated temperatures. Although temperature is known to influence Pst urediniospore germination, the molecular mechanisms underlying temperature sensitivity during this process remain poorly understood. In this study, we combined histological examinations with transcriptome sequencing across multiple temperature regimes to identify temperature-responsive genes and characterize the predicted protein association networks in Pst. Urediniospore germination of three Pst races was tested; stronger heat tolerance was observed in CYR34-8 with 67.40–77.40% germination at 9–16 °C. RNA-Seq analysis identified 89 differentially expressed genes (DEGs) associated with temperature sensitivity, and their expression patterns were validated by qRT-PCR. The DEGs were significantly enriched in ubiquinone and other terpenoid-quinone biosynthesis, glycan degradation, and longevity-regulating pathways. Among these DEGs, genes annotated as encoding an ABC transporter, malate dehydrogenase, Hsp70, and a class 3 lipase were identified as putative hub genes in the predicted protein association network and may be associated with the coordination of temperature responses and metabolic processes.
Fei Tao, Hong Han, Hong Tao et al.· Journal of Fungi· 0 citations
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