Aug 2026· 3 Biotech· Vol 16· 0 citations· 114 references
Medicine
TL;DR
This is the first report describing Bacillus stercoris as a multifunctional endophytic biocontrol and plant growth-promoting bacterium associated with taro, highlighting their potential as eco-friendly bioinoculants for the sustainable management of taro leaf blight.
Among 14 endophytic isolates, Bacillus subtilis LS1-5 showed the strongest antifungal activity by dual culture assay, and exhibited a broad array of plant growth-promoting and environmental resilience traits.
D. Nguyen, Dong Tang Vien, Ngoc-Minh Truong Bui et al.· IOP Conference Series: Earth...· 0 citations
This study introduces L. aquatilis strain MC3 as an emerging candidate for bioinoculant development and one of the first reports for identification of L. aquatilis as multifunctional PGPR from Himalayan ecosystems.
S. Devi, Riya Chandel, D. Thakur et al.· Frontiers in Systems Biology· 0 citations
Fusarium solani is a major soil-borne pathogen responsible for root rot diseases, posing a significant threat to agricultural productivity. The development of environmentally sustainable alternatives to chemical fungicides is therefore urgently needed. This study evaluated the antifungal activity of fermentation broths and extracellular metabolites derived from Tilletia laevis fungi at different developmental stages against F. solani under in vitro conditions. Six test agents, including carbendazim (one positive fungicide control), caffeic acid, phenylacetylglycine, and 6-hydroxypyridine-2-carboxylic acid (candidate bioactive metabolites), were evaluated for mycelial growth inhibition, EC50 values, and physiological responses. All treatments exhibited concentration-dependent inhibitory effects, with carbendazim showing the highest activity (EC50 = 21.26 mg L−1). Among the metabolites, 6-hydroxypyridine-2-carboxylic acid and phenylacetylglycine demonstrated notable antifungal efficacy, particularly at higher concentrations. Fermentation broths from the promycelial stage showed stronger inhibition than those from the teliospore stage, indicating stage-specific metabolite activity. Biochemical analyses of F. solani mycelia revealed significant changes in soluble protein, soluble sugar, and antioxidant enzyme activities (SOD and POD), suggesting that antifungal effects are mediated through metabolic disruption and oxidative stress. These findings highlight the potential of T. laevis-derived metabolites as eco-friendly biofungicides and provide a theoretical basis for sustainable management of soil-borne diseases. Further studies are required to identify active compounds and validate their efficacy under field conditions.
De-Lai Chen, Shirong Ma, Wei-Wei Zhang et al.· Journal of Fungi· 0 citations
India contributes 26% of global banana production, yet cultivation is severely threatened by Fusarium wilt (Fusarium oxysporum f. sp. cubense, Foc), necessitating sustainable, eco-friendly management strategies. This study evaluated the biocontrol potential of endophytic bacteria isolated from Foc-resistant and -susceptible banana cultivars. Isolates from the pseudostem, corm, and root of the resistant cultivar showed significantly greater inhibitory activity than those from the susceptible cultivar, underscoring the role of host genotype in shaping functionally competent endophytic communities. Among all isolates, Bacillus sp. from the corm of cv. Rose (AB) showed the highest mycelial inhibition of Foc (70.37–76.54%) in vitro. GC-MS-based metabolite profiling revealed a chemically diverse array of secondary metabolites, fatty acid esters, steroids, terpenoids, siloxanes, and nitrogenous compounds. Corm-associated endophytes exhibited membrane-disruptive and cytotoxic activity, while root-associated endophytes contributed protective, defense-modulatory effects. Halomonas sp. RoRo2 and B. subtilis RoC1 showed the highest inhibition in both agar-well and in planta assays, with unsaturated fatty acids and organic acid derivatives implicated as key antifungal effectors acting through multiple biochemical pathways. These findings identify metabolically versatile endophytes as promising candidates for developing efficient, environmentally compatible bioinoculants as sustainable alternatives to chemical control of Fusarium wilt in banana.
D. P. Mohite, Kavino Mathiyazhagan, Nakkeeran Sevugapperumal et al.· Pathogens· 0 citations
The present study is aimed at evaluating biocontrol and growth-promoting potential of volatile organic compound (VOC) producing bacterial endophytes against Sclerotinia sclerotiorum in Helianthus annuus L. Two isolates SEB6 and SEB23, exhibiting fungal growth inhibition of 23.75 and 20.0%, respectively, in a dual-plate assay and 76.5 and 35.4%, respectively, in broth were identified by morphological features, biochemical tests, matrix-assisted laser desorption/ionization time of flight mass spectroscopy and 16S rRNA gene sequencing as Bacillus safensis and Serratia liquefaciens. Scanning electron microscopy revealed that fungal growth was inhibited by bacterial antagonists by rupturing, breaking, and shrinking of fungal hyphae. Additionally, the antagonists exhibited phosphate solubilization, and auxins, ammonia, and lipase production. Both strains produced various volatile organic compounds as detected by gas chromatography–mass spectrometry, including 26 VOCs in Bacillus safensis SEB6 and 16 in Serratia liquefaciens SEB23. The compounds 1-docosene, isopropyl myristate, 1,2-benzenedicarboxylic acid, octadecene, diethyl phthalate, glycerin, triacetin, and tetradecane were the common VOCs produced by both the strains. Colonization of plant tissues, as evident by scanning electron microscopy analysis, validated the endophytic nature of both the strains. The pot trial revealed that the combined application of both the strains decreased the disease severity by 88.9% and significantly improved the vigor index, plant growth, total phenol content, and chlorophyll content over control treatment. The maximum increase in N, P, and K contents of 1.73, 0.54, and 6.8%, respectively, was obtained with the treatment BsSEB6 + SlSEB23 in the presence of the fungal pathogen. To the best of our knowledge, this paper for the first time reports the production of 3-pentadecylphenol by the antagonistic Bacillus safensis strain.
R. Kaur, Pratibha Vyas, A. Alsohim· ACS Agricultural Science &am...· 0 citations
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