CRISPR-AMPED achieves attomolar-level sensitivity, surpassing ELISA by over three orders of magnitude and outperforming existing immunoassays and CRISPR/Cas-based protein detection systems.
The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) system enables sensitive and specific biomolecular detection due to its programmability, high fidelity, and signal amplification. Herein, a novel universal CRISPR/Cas12a-G4 DNAzyme-TMB (Cas-GT) enzymatic biosensi...
Huisi Qin, Jing-Xuan Liu, Yang Luo et al.· In Analysis· 0 citations
The design principles and working mechanisms of cascaded CRISPR strategies, encompassing Enzyme-Coupled cascades, Multi-effector class 2 CRISPR cascades, and Type III CRISPR-mediated cascades are introduced, including integration with digital detection platforms and AI-assistant algorithms are discussed.
Ruo-Nan He, Lu Miao, Rui-Jie Deng et al.· Biosensors & bioelectronics· 0 citations
CATNAP (Cas trans -nuclease detection of amplified products), a method that integrates isothermal linear DNA amplification with Cas12a detection in a single reaction, bridges the sensitivity gap in CRISPR diagnostics while maintaining simplicity, making accurate disease detection more accessible in resource-limited setti...
Selma Sinan, Remy M. Kooistra, Karunya Rajaraman et al.· 0 citations
A modular fluorescence signal transduction platform integrating aptamer-functionalized magnetic beads, lanthanide metal-organic frameworks, and CRISPR-Cas12a-mediated collateral cleavage amplification for sensitive cTnI detection, providing a universal strategy to expand CRISPR-Cas systems toward non-nucleic acid targe...
Yongzhen Liu, Long Yu, Yongjin Shen et al.· Analytical Chemistry· 0 citations
This review summarizes the structural and mechanistic features of representative CRISPR and Ago effectors and discusses design strategies for sensitive, specific, and multiplexed imaging of genomic loci, extrachromosomal DNA, and endogenous RNA in living cells.
Mycobacterium avium subsp. paratuberculosis (MAP), the causative agent of Johne's disease in ruminants, has attracted increasing attention because of its potential zoonotic relevance. Sensitive molecular detection of MAP typically relies on nucleic acid amplification to overcome low pathogen abundance; however, amplifi...
Shou-Yan Wu, Xiu-Mei Mu, Zhi-Juan Wu· In Analysis· 0 citations
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