Aug 2026· Journal of Agricultural and Food Chemistry· Vol 74 32, pp.
25660-25673
· 0 citations· 44 references
Medicine
Abstract
Aflatoxin M1 (AFM1) in milk poses significant health risks due to its carcinogenicity and resistance to thermal processing. Here, we applied a computer-aided hapten design strategy integrating density functional theory, molecular docking, and molecular dynamics simulations to engineer four AFM1 haptens with tailored spacer arms (C2, C4, C6, and phenyl). A high-affinity monoclonal antibody (10C1, IC50 = 0.015 ng/mL) was generated. Based on this antibody, an icELISA (LOD = 0.0017 ng/mL, meeting EU standards of 0.05 ng/mL) and a gold nanoparticle-based LFIA (LOD = 0.3 ng/mL, meeting China/US/Russia standards of 0.5 ng/mL) were developed for laboratory and on-site detection, respectively. This strategy facilitates rational hapten optimization and efficient generation of high-affinity antibodies for AFM1 monitoring.
Fluroxypyr (FLU) is a widely used synthetic auxin-type herbicide. FLU residues in agricultural products pose potential risks to the environment and human health. Therefore, the development of rapid and sensitive fluroxypyr detection methods is highly important. In this study, a monoclonal antibody, 11D8 (mAb-11D8), cap...
Si Chen, Pei-Pei Li, Chen-Xi Guo et al.· Analytical Methods· 0 citations
The health risks posed by chloroneb (CHL), a representative fungicide, in agricultural products and the environment necessitate rapid detection methods. Here, computational chemistry-aided rational hapten design yielded a high-performance monoclonal antibody against CHL (IC50 = 2.63 ng/mL), guided by analysis of its...
Feng He, Ling-Ling Guo, Mao-Zhong Sun et al.· Journal of Agricultural and...· 0 citations
Florfenicol (FF), a broad-spectrum amide antibiotic widely used in livestock, poultry, and aquaculture, poses potential threats to food safety and public health due to its residual accumulation. In this study, a novel homogeneous immunoassay based on Amplified Luminescent Proximity Homogeneous Assay (AlphaLICA) technol...
Dian Li, Canlong Yan, Mengwei Li et al.· Analytical Methods· 0 citations
Aflatoxin M1 (AFM1), the 4-hydroxylated metabolite of aflatoxin B1, is excreted in the milk of lactating animals fed AFB1-contaminated feed and is of public-health concern because of its well-documented thermal stability during dairy processing. In this study, a matrix-specific extraction, immunoaffinity clean-up, and...
Prince Dhanda, K. Gandhi, Raghu Hrikyathahalli Vishweswaraiah et al.· Journal of Food Science· 0 citations
Phthalate esters (PAEs) are frequently detected in edible oils, necessitating rapid detection methods. However, the small molecular size and high structural similarity of PAEs make it challenging to develop antibodies that combine analytical sensitivity with group-selective recognition. Herein, a monoclonal antibody (m...
