Jul 2026· Physiology and Molecular Biology of Plants· Vol 32, pp. 1955 - 1973· 0 citations· 81 references
Medicine
TL;DR
This work provides a foundational resource for C. diurnum for future pharmaceutical and biotechnological applications, enables the discovery and functional characterization of candidate genes, supports metabolic engineering strategies to enhance valuable terpenoids, and offers avenues to mitigate toxic alkaloid accumulation.
Findings reveal tissue-specific metabolite accumulation and key genes involved in MIA biosynthesis in N. cadamba that provide valuable insights into specialized metabolism and establish a foundation for future metabolic engineering and functional genomics studies.
Divya Selvakumar, G. Ramalingam, Suganya Balan et al.· Molecular Biology Reports· 0 citations
Key genes in the mevalonate (MVA) pathway were upregulated in roots, facilitating metabolic crosstalk with the methylerythritol-phosphate pathway and enhancing terpenoid skeleton synthesis.
This review compares the historical and methodological trajectories that have shaped IA and AA pathway elucidation, from compound isolation, radiotracer experiments, and biochemical inference to transcriptomics, metabolomics, functional enzymology, isotope-guided active-tissue identification, regulatory studies, and heterologous pathway reconstruction.
Mateo Peña-Morales, J. D. Vega-Páez, Natalie Cortes et al.· Plants· 0 citations
A decoupled evolutionary strategy between upstream precursor supply and downstream diversification in triterpenoid biosynthesis in R. roxburghii is suggested, providing a valuable genomic resource and a prioritized set of candidate genes for future metabolic engineering and quality improvement of this functional fruit crop.
Hua-Yue Zhang, Yue-Zhu Wang, Ming-Jie Li et al.· Plant physiology and biochem...· 0 citations
These findings collectively support flavanone 3-hydroxylase (F3H) as a candidate regulatory node governing organ-specific flavonoid partitioning in Rhododendron yedoense var.
Huperzine A (HupA) is a natural Lycopodium alkaloid known for its potent neuroprotective properties through the inhibition of acetylcholinesterase. Nevertheless, the limited understanding of its biosynthesis restricts its broader application. This study integrates full-length and second-generation transcriptomes with the quantification of HupA and its precursor, huperzine B, across various tissues of Huperzia serrata, the primary source plant. By employing phylogenetic clustering, expression profiling, and correlation analysis between gene expression and metabolite abundance, we identified 71 candidate genes from seven enzyme families potentially involved in the synthesis of the HupA backbone, including lysine/ornithine decarboxylases, copper amine oxidases (CAOs), chalcone synthases, and cytochrome P450 monooxygenases. Additionally, 28 genes from two families were identified for modification reactions, specifically 2-oxoglutarate/Fe(II)-dependent dioxygenases and caffeoyl shikimate esterases. Comparative analysis between young and mature leaves revealed 3801 genes with higher expression in young leaves, with 84 showing a high correlation with HupA content across seven families. Protein–protein interaction network analysis indicated possible interactions with transcription factors from the MYB, NF-YC, GRAS, ERF, BHLH, and SAP families. Functional validation of two candidate CAOs in planta confirmed their catalytic roles in amine/alkaloid metabolism. This study provides a theoretical foundation and a set of candidate genes for elucidating the biosynthetic pathway of HupA and related alkaloids in H. serrata.
M. Lei, Jing Wang, Cui Li et al.· Horticulturae· 0 citations
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