Jul 2026· Journal of Integral Sciences· 0 citations· 10 references
TL;DR
The extensive molecular identification, genetic sequencing, and evolutionary positioning of the bacterial isolate ANU-27, which exhibited superior broad-spectrum antimicrobial efficacy during initial phenotypic screenings are highlighted.
Abstract
Estuarine mangrove environments are unique, highly dynamic habitats harboring a rich and unexplored diversity of bioactive actinobacteria. In this study, we highlight the extensive molecular identification, genetic sequencing, and evolutionary positioning of the bacterial isolate ANU-27, which exhibited superior broad-spectrum antimicrobial efficacy during initial phenotypic screenings. Genomic DNA was successfully extracted using the CTAB-lysozyme methodology, yielding634±32 µg/gof high-integrity DNA with a distinct genomic G+C content of 55.1% calculated via thermal denaturation midpoint (Tm= 92.3C).The 16S rRNA gene locus was targeted and amplified via PCR utilizing 27F and 1492R universal primers, generating a sharp ~1500 bp amplicon. Bidirectional Sanger sequencing resolved a definitive sequence spanning 1177 nucleotides with a high ribosomal G+C ratio 59.13%. Comprehensive homology mapping via NCBI BLASTn paired with a1000-replicate Maximum Parsimony cladogram clustered the strain with 100% sequence identity( E-Value = 0.0) within the genus Streptomyces, revealing an immediate phylogenetic node shared with Streptomyces maritimus strain SBK2-IR9 and Streptomyces rochei strain ABU8. These findings firmly clarify the taxonomic assignment of strain ANU-27, spotlighting its high secondary metabolic and biotechnological promise.
Members of the phylum
Actinomycetota
are widely distributed across diverse environments and are well known for their metabolic versatility and capacity to produce bioactive compounds. In this study, strain ZE1316R2Aᵀ was isolated from the saline water collected from Lake Zima (Morocco) and subjected to comprehensive polyphasic taxonomic characterisation. Phylogenetic analysis based on the 16 S rRNA gene placed strain ZE1316R2Aᵀ within the genus
Streptomyces
, showing highest sequence similarity with
S. albidoflavus
DSM 40,455
T
(99.71%). However, genome-based indices, including average nucleotide identity (ANIb = 94.84%, ANIm = 96.09%) and digital DNA-DNA hybridization (dDDH = 64.9%), supported its distinction as a separate species. The draft genome (7.41 Mb; G + C = 73.26 mol%) comprises 6,464 coding sequences and reveals the presence of strain-specific genomic regions and biosynthetic gene clusters. Comparative analyses highlighted both a conserved core genome and a substantial accessory genome component, reflecting genomic differentiation relative to closely related taxa. Phenotypic and chemotaxonomic characteristics were consistent with assignment to the genus
Streptomyces
, while supporting its differentiation at the species level. Based on the combined genomic, phenotypic, and chemotaxonomic evidence, strain ZE1316R2Aᵀ represents a novel species of the genus
Streptomyces
, for which the name
Streptomyces zimensis
sp. nov., is proposed. This study expands current knowledge of
Streptomyces
diversity associated with saline environments and highlights the genomic diversity present within closely related taxa. The type strain is ZE1316R2Aᵀ (= CCMM B1331
T
= DSM 120541
T
).
E. Oubassou, Soukaina Oudchaira, V. Cognat et al.· Annals of Microbiology· 0 citations
The genome provides a valuable resource for investigating anaerobic carbon metabolism, acetate biosynthesis, sulfur-associated pathways, and the ecological adaptation of Fusobacterium-related bacteria in coastal sediment ecosystems.
M. Prathaban, R. Prathiviraj, M. Sobanaa et al.· Data in Brief· 0 citations
The phylogenetic affiliations of anaerobic gut fungi (Neocallimastigomycota) are typically evaluated using single-gene markers. However, this approach often fails to resolve relationships between closely related lineages. To address this issue and identify alternative markers, we created a curated database comprising the complete ribosomal operon sequences of 156 isolates, representing 20 of the 22 recognized genera and two new genus-level clades. Using long-read sequencing, we obtained ~9 kbp operon sequences and developed a robust analysis pipeline. Incorporating both coding genes and non-coding regions (excluding IGS1) improved phylogenetic resolution. This phylogenetic approach successfully resolved the Cyllamyces and Caecomyces clades (hard-to-distinguish genetically), as well as seven analysed Piromyces species. We also scanned the operon for markers that are suitable for short-read sequencing platforms, with the aim of enhancing biodiversity and phylogenetic studies. Notably, the ETS1 genetic region also enabled the distinction between these lineages, indicating its phylogenetic value within the ribosomal operon. The resulting database is a valuable resource for expanding and strengthening phylogenetic frameworks.
Diana Young, Katrin Stüer-Patowsky, Liren Huang et al.· IMA Fungus· 0 citations
Two B. licheniformis strains isolated from extreme environments are isolated from the Five Sisters hot spring in Yellowstone National Park and whole-genome sequencing reveals an open pangenome of 10,374 genes, providing a genomic foundation for future exploration of novel bioactive compounds with potential applications in drug discovery, agriculture, and biotechnology.
O. Elsakhawy, Mohamed A. Abouelkhair, S. Kania· Frontiers in Bioinformatics· 0 citations
Non-tuberculous mycobacteria (NTM) are increasingly recognized as opportunistic pathogens, yet accurate species identification remains challenging. In this study, two clinical isolates, 19-857T and 20-525, obtained from patients with suspected pulmonary tuberculosis in Beijing, were characterized using a polyphasic approach integrating phenotypic, biochemical, chemotaxonomic, and genomic analyses. Phylogenetic analysis based on 16S rRNA gene sequences placed both strains within the genus Mycobacterium, with highest similarity to M. peregrinum DSM 43271T and M. arcueilense DSM 46715T. However, whole-genome comparisons via average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) yielded values of 85.8–94.1% and 29.1–54.3%, respectively, against their closest relatives—well below the species delineation thresholds (95–96% for ANI; 70% for dDDH)—while the two isolates shared 98.8% ANI and 90.1% dDDH, confirming their co-assignment to a single novel species. Genomic analysis further revealed a repertoire of genes potentially associated with virulence, including glycopeptidolipid biosynthesis, mce operons, ESX secretion systems, and mycobactin synthesis, alongside antimicrobial resistance determinants against rifampicin, tetracyclines, and β-lactams. Mobile genetic element analysis also uncovered extensive insertion sequences and transposon-related elements with differential distribution between the two strains. Based on the combined evidence, we propose Mycobacterium shunyiense sp. nov., with strain 19-857T (=CGMCC 38906T) as the type strain. This discovery expands the understanding of NTM diversity and provides genomic reference data for accurate clinical identification.
Yang Deng, Xinyu Yang, Nenhan Wang et al.· International Journal of Mol...· 0 citations
Based on phylogenetic analyses combined with phenotypic and chemotaxonomic characterization, strains MA13-6T and MA13-13 represent a novel species of the genus Aquipuribacter, for which the name Aquipuribacter aurantiacus sp.
Xia Luo, Mingzhu Zhang, Yumo Li et al.· Systematic and Applied Micro...· 0 citations
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