Leucobacter shiyiweii sp. nov. and Leucobacter fengyongliangi sp. nov., isolated from coal samples in Shanxi Province of China.

Polyphasic and genomic characterisation of Streptomyces zimensis sp. nov., a halotolerant Actinomycetota from the Saline Lake Zima in Morocco

Members of the phylum Actinomycetota are widely distributed across diverse environments and are well known for their metabolic versatility and capacity to produce bioactive compounds. In this study, strain ZE1316R2Aᵀ was isolated from the saline water collected from Lake Zima (Morocco) and subjected to comprehensive polyphasic taxonomic characterisation. Phylogenetic analysis based on the 16 S rRNA gene placed strain ZE1316R2Aᵀ within the genus Streptomyces , showing highest sequence similarity with S. albidoflavus DSM 40,455 T (99.71%). However, genome-based indices, including average nucleotide identity (ANIb = 94.84%, ANIm = 96.09%) and digital DNA-DNA hybridization (dDDH = 64.9%), supported its distinction as a separate species. The draft genome (7.41 Mb; G + C = 73.26 mol%) comprises 6,464 coding sequences and reveals the presence of strain-specific genomic regions and biosynthetic gene clusters. Comparative analyses highlighted both a conserved core genome and a substantial accessory genome component, reflecting genomic differentiation relative to closely related taxa. Phenotypic and chemotaxonomic characteristics were consistent with assignment to the genus Streptomyces , while supporting its differentiation at the species level. Based on the combined genomic, phenotypic, and chemotaxonomic evidence, strain ZE1316R2Aᵀ represents a novel species of the genus Streptomyces , for which the name Streptomyces zimensis sp. nov., is proposed. This study expands current knowledge of Streptomyces diversity associated with saline environments and highlights the genomic diversity present within closely related taxa. The type strain is ZE1316R2Aᵀ (= CCMM B1331 T  = DSM 120541 T ).

Microbacterium altimontanum sp. nov., Microbacterium longlingense sp. nov., and Microbacterium cucumeris sp. nov.: Three New Species of Microbacterium Isolated from Soil in Yunnan Province, China

Three actinobacterial strains, AW10-10T, M8-1T and HGHZ1-2T, were isolated from soil in Yunnan Province, China and analyses of 16S rRNA gene sequences and shotgun-sequenced draft genomes placed them within the genus Microbacterium, well below the accepted species-level thresholds.

Altererythrobacter chaiwopuensis sp. nov., a novel alphaproteobacterial strain isolated from sediment of the Chaiwopu Lake

Abstract Two alphaproteobacterial strains, designated EGI L300112T and EGI L300120, were isolated from sediment collected from Chaiwopu Lake in Xinjiang, China. The taxonomic position of the two strains was determined using polyphasic taxonomic analysis and phylogenomic analysis. Based on 16S rRNA gene sequence similarities, strain EGI L300112T and EGI L300120 were both closely related to Pelagerythrobacter rhizovicinus AY-3RT and shared the highest sequence identities of 96.2% and 96.3%, respectively. However, phylogenetic analysis based on the 16S rRNA gene and genomes clearly demonstrated that the two strains formed a distinct clade with validly published species of the genus Altererythrobacter. Furthermore, the two strains exhibited distinct phenotypic, physiological and genotypic characteristics that differentiate them from other related type species of the genus Altererythrobacter and related species in the family Erythrobacteraceae. Cells of the two strains were aerobic, Gram-stain negative, non-motile and rod-shaped. Optimal growth conditions for EGI L300112T and EGI L300120 occurred on marine agar 2216 at pH 7 at 30 °C. The major respiratory quinone was Q-10. The detected polar lipids of two strains included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine and unidentified phospholipids. The major fatty acids (>10%) were identified as summed feature 3 (C16 : 1ω7 c/C16 : 1ω6 c) and summed feature 8 (C18 : 1ω7 c). The G+C content of strain EGI L300112T and EGI L300120 was 61.2% and 61.4%, respectively. Based on differential phenotypic and genotypic characteristics of the two strains and related species in the genus Altererythrobacter, the two strains should be classified as representing a new species of this genus, for which the name Altererythrobacter chaiwopuensis sp. nov. is proposed. The type strain is EGI L300112T (=MCCC 1K09432T=KCTC 8712T).

Description and genomic characterization of Aquipuribacter aurantiacus sp. nov., isolated from saline lake sediment.

Based on phylogenetic analyses combined with phenotypic and chemotaxonomic characterization, strains MA13-6T and MA13-13 represent a novel species of the genus Aquipuribacter, for which the name Aquipuribacter aurantiacus sp.

Pseudoalteromonas nanhaiensis sp. nov., a Novel Bacterium Isolated from South China Sea

Whole genome sequencing and molecular characterization of two Bacillus licheniformis strains isolated from hot springs in yellowstone ational park

Two B. licheniformis strains isolated from extreme environments are isolated from the Five Sisters hot spring in Yellowstone National Park and whole-genome sequencing reveals an open pangenome of 10,374 genes, providing a genomic foundation for future exploration of novel bioactive compounds with potential applications in drug discovery, agriculture, and biotechnology.