Aug 2026· Biodegradation· Vol 37· 0 citations· 45 references
Medicine
TL;DR
Overall, this integrated bioprocess offers a sustainable solution for nitrate and microbial contamination, supporting environmental protection and public health in line with SDG 3 (Good Health and Well-Being) and SDG 6 (Clean Water and Sanitation).
Hydrocarbon contamination represents a major environmental challenge in oil-producing regions, particularly in arid environments such as Eastern Libya, where natural attenuation processes are severely limited. This study evaluates the effectiveness of a combined nano-bioremediation approach using indigenous bacterial strains (Pseudomonas aeruginosa and Bacillus subtilis) and biosynthesized silver nanoparticles (AgNPs) for the remediation of diesel-contaminated soils collected from the Tobruk region. Laboratory experiments were conducted over a 28-day period under controlled conditions. Hydrocarbon degradation was assessed using gravimetric analysis, and statistical evaluation was performed to compare treatment efficiencies. The results revealed that the combined treatment achieved the highest degradation efficiency (70%), followed by nanoparticles alone (44%) and bacterial treatment alone (36%), whereas the control showed minimal degradation (8%). The enhanced performance of the combined system is attributed to the synergistic interaction between microbial metabolism and nanoparticle-induced improvements in hydrocarbon bioavailability. This study highlights the potential of nano-bioremediation as a sustainable and cost-effective solution for environmental restoration in arid regions.
Faeza Mharb, Lazam A. Abraik, Said Saad· Al-imad Journal of Humanitie...· 0 citations
The most potent strain, Bacillus paralicheniformis UB08 (TISTR 10842), displayed an extraordinary nominal Pb tolerance, with a minimum bactericidal concentration exceeding 9,000 ppm, and alginate bead encapsulation enables rapid and complete Pb removal, offering a promising solution for heavy metal treatment based on physical adsorption and potential biological synergies.
Kaninnut Sangkhum, T. Panich-pat, P. Nimnoi et al.· PeerJ· 0 citations
The effective implementation of bioremediation is often constrained by the stability and delivery of microbial inocula. This study investigated the development and optimization of a hydrocarbon-degrading consortium comprising three indigenous bacterial strains: Rhodococcus ruber JN5.2, Stenotrophomonas acidaminiphila ZB2.1, and Bacillus amyloliquefaciens MD3.3. Phylogenetic analysis confirmed the taxonomic diversity of the members, while cross-streak assays established total biological compatibility, enabling stable coexistence. In synthetic wastewater containing 5% (v/v) oil, the bacterial consortium achieved a total petroleum hydrocarbon (TPH) removal efficiency of 78 ± 6% within 14 days. This performance significantly exceeded that of individual strains (51-54%), supported by a Synergy Index (SI) of 1.48 and a peak biomass density of 8.6 ± 0.9 × 10⁸ CFU/mL. To enhance practical applicability, freeze-drying conditions were optimized. Harvesting biomass at the 24-hour growth phase and utilizing a protective formulation of 10% skim milk, 5% sodium glutamate, and 20% bentonite preserved bacterial viability at ≥ 9.3 log₁₀ CFU/mL after one month of storage. The consortium was evaluated in three formulations: liquid culture, freeze-dried powder (FDO-VN25), and alginate-immobilized beads. Validation using real car-washing wastewater (TPH ≤ 100 mg/L) revealed that while all the formulations improved degradation, the alginate-immobilized system consistently exhibited the highest stability and efficacy, achieving over 60% TPH removal after 14 days. These results demonstrate that integrating a native multi-species consortium with optimized lyophilization and alginate encapsulation provides a robust microbial solution for treating low-strength petroleum-contaminated effluents in complex environments.
Unknown authors· Journal of Tropical Science...· 0 citations
Pharmaceutical and personal care products (PPCPs) residues pose ecological and health risks due to their persistence and promotion of antibiotic resistance genes (ARGs) dissemination. Here, we constructed a synthetic microbial community (SMC) comprising two bacterial strains (Sphingopyxis sp. GC21 and Ochrobactrum sp. TCC-2) and one fungal strain (Apiotrichum sp. IB-1) for the enhanced simultaneous removal of three representative PPCPs: chloramphenicol (CAP), triclocarban (TCC), and naproxen (NPX) in simulated wastewater (SW) and real wastewater (RW). Under aerobic conditions, the removal efficiencies (REs) reached 97.83% for CAP, 68.19% for TCC, and 85.34% for NPX within 8 h in SW, while all REs exceeded 70% in RW. Under anoxic conditions, REs exceeded 60% for CAP, TCC and NPX both in SW and RW. The relative abundance of introduced Sphingopyxis was 6.45%, Ochrobactrum was 0.23%, and Apiotrichum was 51.06%, confirming successful colonization of them in the SMC reactor. They were responsible for the nitro-reduction and amide bond hydrolysis of CAP, two amide bonds hydrolysis of TCC, and O-demethylation and oxidation of NPX, which could detoxify these pollutants and promote cooperative degradation by indigenous microbial communities. Furthermore, SMC alleviated the selection pressure imposed by pollutants, thereby reducing the enrichment of mobile genetic elements and pathogens and suppressing the dissemination of ARGs. This study highlighted that SMC bioaugmentation provided an effective and sustainable strategy for mitigating complex PPCPs pollutions and controlling the spread of ARGs.
Ting Xu, Hui Yun, Yuru Zhao et al.· Journal of Hazardous Materia...· 0 citations
The present study addresses the problem of pollution in the Sumgayit River, which has been
exposed to technogenic impacts for many years, and proposes new approaches to enhance its selfpurification capacity. To purify river water from organic and inorganic pollutants, the feasibility of
selecting active strains from the native microbiota and applying them in bioremediation was
investigated. According to the research results, river waters subjected to anthropogenic pollution
exceeded the maximum permissible concentrations (MPCs): the phenol concentration was 0.50 mg/L
(MPC = 0.001 mg/L), while the copper concentration was 0.96 mg/L (MPC = 0.01–0.1 mg/L). It was
established that the active microbial strains isolated from these water samples belonged to the genera
Rhodococcus, Penicillium, and Aspergillus. The obtained results demonstrated that the fungal strains
were more effective than the bacterial strain. Under the influence of Rhodococcus sp., the phenol
concentration decreased by 62%; Penicillium sp. achieved a 100% reduction, while Aspergillus sp.
reduced the phenol concentration by 86%. The copper concentration decreased by 58.4% with
Rhodococcus sp., 64.8% with Penicillium sp., and 68.7% with Aspergillus sp. These results indicate
that these microorganisms are effective candidates for application in bioaugmentation.
A. Gasimova, K. Bayram, S. Nadjafova et al.· Scientific Works· 0 citations
The textile industry generates large volumes of dye-laden effluents that pose risks to aquatic ecosystems and human health. This study evaluates the bioaccumulation of Reactive Black 5 (RB5) using Candida boidinii and Kluyveromyces marxianus yeast strains. Key operational parameters including initial pH, incubation time, dye concentration, and microbial growth were systematically investigated. In addition, the effects of low-cost agricultural wastes-carrot pomace (CP), industrial tea waste (ITW), and pumpkin pomace (PP)-as supplementary substrates were compared. Results showed that CP was the most effective substrate, with 100 g/L CP significantly enhancing RB5 removal. At pH 5.0 and 50 mg/L RB5, removal efficiencies reached 96.88% for C. boidinii and 81.76% for K. marxianus with CP, compared to 72.90% and 65.07% with PP. Although removal efficiency decreased at higher dye concentrations, the maximum dye uptake capacity (qm) increased with initial RB5 concentration, reaching 52.52 mg/g for C. boidinii and 33.23 mg/g for K. marxianus over the tested range (≈50-600 mg/L). To our knowledge, this is the first report demonstrating RB5 removal by these yeasts using CP as a low-cost substrate. Overall, CP significantly improves yeast-based RB5 bioaccumulation and represents a promising, sustainable option for treating dye-contaminated wastewater.
