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A field deployable duplex RPA-CRISPR/Cas12a assay for rapid and sensitive detection of African swine fever virus

P. Madhankumar G. Sharma K. G. Sai Balaji Kanchan Singh Samridhi Dixit Shivali Khandelwal Patil Ranjeet Mahesh R. Manju K. Mathesh P. K. Gupta Ravi Kant Agrawal Sonalika Mahajan
Sep 2026 · Archives of Virology · Vol 171 · 0 citations · 65 references
Medicine

TL;DR

A rapid, field-deployable recombinase polymerase amplification (RPA)-CRISPR/Cas12a assay for the detection of African swine fever virus using both fluorescence- and lateral flow strip (LFS)-based readouts shows 100% analytical specificity against other porcine viral pathogens and strong agreement with real-time PCR.

DOI PubMed Semantic Scholar
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