The "OFF-ON" PersL property of MYG-S was applied to develop a physi-luminescence assay method for H2O2, glucose, and glucose oxidase in serum, and high-throughput photographic luminescence analysis was realized with accuracy and repeatability.
Abstract
Chemi- or bioluminescence assays have broad applications in biomedical analysis, yet they still suffer from a flash effect or difficulty in maintaining time synchronization during high-throughput assays. Persistent luminescence (PersL) nanoprobes are able to emit photons for a rather long time without any chemical reactions and have promising applications in developing a physi-luminescence assay. Herein, SiO2/Zn2SiO4:Mn,Yb,Ge nanoparticles (MYG, Zn/Si/Mn/Yb/Ge molar ratio, 2/15/0.003/0.060/0.100) were synthesized with PersL properties at 525 nm. Mesoporous silica nanoparticles were used as a nanotemplate to control the size and morphology, which allowed for gram-scale production (1.1277 g). Ge was found to be an efficient codopant to realize the enhanced PersL of MYG. A hydrophobic azo dye, Sudan III, was loaded onto the MYG surface to form a quenched MYG-S nanoprobe. Under 254 nm UV illumination, Sudan III was oxidized by H2O2, and PersL was recovered. The "OFF-ON" PersL property of MYG-S was applied to develop a physi-luminescence assay method for H2O2, glucose, and glucose oxidase in serum. As the PersL of the samples in a 96-well plate was activated by simultaneous UV illumination rather than by adding chemicals, high-throughput photographic luminescence analysis was realized with accuracy and repeatability. Our research indicates the promising applications of PersL nanoprobes in developing a physi-luminescence assay.
Metal halide perovskite quantum dots (PQDs) exhibit strong optical absorption, high photoluminescence quantum yields, spectrally narrow and adjustable emission, and high defect tolerance. However, enhancing their stability in aqueous solution and improving their analytical performance remain critical challenges for the development of sensors for biological and chemical analytes. Stability and reproducibility in aqueous and physiological media are paramount for functional biosensing and bioimaging. Thus, most studies have focused on the analysis of chemicals in food safety, chemical product quality control, and industry process monitoring, whereas molecular diagnostics and cellular imaging have mainly remained in a proof‐of‐concept stage. In this study, we review applications of PQDs in luminescence sensing and imaging. After a brief introduction of PQD structure, synthesis, stability, and optical properties, we focus on applications of PQD‐based sensing via photoluminescence, chemiluminescence, and electroluminescence, as well as their use in luminescence imaging. We discuss current challenges, potential solutions, and future opportunities for developing PQD‐based sensing platforms.
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Engineering light-responsive nanoparticles (NPs) with tailored nonlinear optical properties is opening new opportunities in precision biophotonics. This article highlights gold nanoparticles (AuNPs) and carbon dots (CDs) as complementary nanoscale platforms for multiphoton bioimaging, photodynamic therapy, sensing, and theranostic applications. Key methods for probing nonlinear optical behaviour, including Z-scan, two-photon excited fluorescence spectroscopy, and hyper-Rayleigh scattering, are first outlined. The discussion focuses on AuNPs, covering bioinspired synthesis, biofriendly post-processing, and representative functions in antimicrobial photodynamic inactivation, plasmonic catalysis, sensing, and nonlinear optical readout. Special attention is also given to lyotropic liquid-crystalline systems (LLCs), particularly DNA-based mesophases and myelin figures, as biomimetic environments that enable NP organisation, polarisation-sensitive studies, and photothermal control of local order of LLCs. CDs are presented more concisely as an earth-abundant complementary platform whose multiphoton responses can be tuned through internal structure and surface chemistry, with emerging relevance in biomolecular environments and soft-matter imaging. Overall, this review connects materials design with optical function and biological performance, and points to future opportunities in deeper-window operation, hybrid nanostructures, and translational biophotonics.
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Real-time monitoring of toxic triethylamine (TEA) at room temperature remains a significant challenge for metal oxide chemiresistors due to their typically high-power consumption and thermal requirements. This work presents a highly responsive, visible-light-activated gas sensor based on ZnO nanoparticles (25.5 nm crystallites) functionalized with Cu-porphyrin (CuTPPCOOH). By leveraging the visible-light-harvesting properties of the porphyrin integrated into a nanoscale organic/inorganic heterojunction, the hybrid sensor operates efficiently at room temperature, completely eliminating the need for thermal heating. Owing to the nanoscale dimensions of the oxide domains, which maximize boundary depletion region modulation, the optimized device exhibits an ultralow theoretical limit of detection of 0.4 ppb and a superior response (162 toward 10 ppm TEA), significantly outperforming pristine ZnO nanostructures and current state-of-the-art sensors. The sensing enhancement is driven by a synergistic photoinduced charge transfer at the functionalized nanometer-scale interface, which maximizes sensing response under visible light illumination. With demonstrated high selectivity, excellent moisture stability, and rapid response dynamics, this nanoengineered hybrid architecture offers a robust, low-power solution for next-generation environmental safety networks and portable TEA monitors.
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