Evolution trajectory of auxin response factor across Triticeae species underpin candidate genes for the regulation of salt/alkali stress responsive networks
It is demonstrated that underutilized genome evolution data aids gene mining in complex crop genomes, providing novel genetic resources for wheat salt/alkali tolerance breeding and insights into auxin-mediated stress adaptation mechanisms.
It is demonstrated that heterologous expression of TksPLATZ1, TksPLATZ2 and TksPLATZ7 localize to the cell nucleus and act as transcriptional activators and repressors, respectively, which enhances the tolerance of Arabidopsis to salt and osmotic stress.
Jinxian Chen, Wenhao Wu, Ming-Hua Luo et al.· Phytochemistry· 0 citations
A hormone- and anthocyanin-centered framework is proposed for understanding the apparent negative association between salt/drought tolerance and thermotolerance in B. rapa and suggests that hormone-directed anthocyanin metabolism may contribute to the negative association between osmotic tolerance and thermotolerance.
Mei Zheng, Pei-Rong Li, Xiao-Yun Xin et al.· Frontiers in Plant Science· 0 citations
Soil salinization severely limits forage crop productivity, yet the regulatory networks that govern salt stress adaptation in alfalfa, a moderately salt-tolerant leguminous forage, remain largely unexplored. Here, we examined the physiological and transcriptomic dynamics of alfalfa leaves under 200 mM NaCl stress across three time points. Salt stress induced a progressive elevation of the Na+/K+ ratio, biphasic activation of antioxidant enzymes and concurrent accumulation of malondialdehyde. Time-course RNA-seq analysis identified 3631 differentially expressed genes (DEGs) and 132 core salt-responsive transcription factors (TFs). Pathway and functional annotation analyses indicated that these DEGs were prominently involved in cell wall biogenesis, redox homeostasis, and the carotenoid biosynthesis pathway, with carotenoid accumulation strongly activated under salt stress. Using weighted gene co-expression network analysis (WGCNA), nine distinct co-expression clusters were constructed. Notably, the brown module, which showed a positive correlation with Na+ accumulation and the Na+/K+ ratio, was significantly enriched in the plant hormone signal transduction pathway, within which 72.7% of the enriched genes belonged to the TIFY family. Among them, a core hub gene, MsTIFY11B, was isolated for functional characterization. Subcellular localization demonstrated that MsTIFY11B is exclusively localized to the nucleus. Heterologous expression in yeast showed that MsTIFY11B overexpression enhanced tolerance to salinity and alkalinity, whereas it conferred negligible protection against mannitol-induced drought stress. Taken together, our findings provide a comprehensive temporal framework of the alfalfa transcriptomic response to salinity and suggest that MsTIFY11B may contribute to salt–alkali tolerance, making it a promising candidate for further functional characterization and potential application in the development of stress-adapted alfalfa varieties.
Lin Cheng, Yan-Feng Liu, Qing-Chun Liu et al.· Plants· 0 citations
An evolutionary and transcriptional atlas of the wheat TaBSK family is delivered and candidate genes for functional validation and molecular breeding toward salt-tolerant wheat varieties are provided.
Yong-Tao Zhao, Jun-Sen Wang, Zhong-Zhou Zhang et al.· Current Issues in Molecular...· 0 citations
FaNAC6 was selected for its strong induction in leaves and roots in response to drought and salinity, as well as under oxidative stress and ABA, and was associated with the upregulation of genes involved in photosystems, electron transport and carbon fixation.
Facundo Spadoni-Revol, M. D. Moreno-Recio, Sara Aguado-Delgado et al.· International Journal of Mol...· 0 citations
AP2/ERF (APETALA2/ethylene-responsive factor) represents one of the largest transcription factor superfamilies in plants, playing crucial roles in regulating plant growth and development as well as responding to abiotic stresses. Investigating the functions of maize (Zea mays L.) AP2/ERF family genes will provide novel genetic resources for maize genetic improvement. In this study, the AP2/ERF transcription factor superfamily member ZmEREB54 (GRMZM2G020054, Gene ID: 100,278,463) was cloned from maize and was systematically analyzed functionally. The full-length CDS of ZmEREB54 gene was 561 bp, encoding 186 amino acids with a typical AP2/ERF conserved domain. Its promoter region contained cis-acting elements associated with responses to various abiotic stresses and hormones. Maize expression pattern analysis revealed that ZmEREB54 was highly expressed in V12 roots, with significant expression changes under osmotic stress, drought, high salinity, and treatments with abscisic acid (ABA) and jasmonic acid (JA). Phenotypic analysis showed that transgenic Arabidopsis thaliana over-expressing ZmEREB54 exhibited significantly longer roots compared to wild-type plants under high salinity, drought, osmotic stress, and hormone treatments (JA, ABA). Stress-responsive marker genes RD29A and RD22 were upregulated in the transgenic A. thaliana lines. The significantly decreased malondialdehyde (MDA) accumulation and markedly increased peroxidase (POD) activity in transgenic A. thaliana further demonstrate the improvement of its stress tolerance. Yeast two-hybrid (Y2H) assays revealed an interaction between ZmEREB54 and ZmMADS24.6, suggesting potential cooperative regulation of ZmEREB54 and ZmMADS24.6 in maize root development and stress responses. This study establishes a solid foundation for further clarifying the biological functions and molecular mechanisms of ZmEREB54 in regulating maize root growth and development, as well as responding to drought and salt stresses.
Yu-Qian Gao, Jun-Xia Wang, D. Zheng et al.· BMC Plant Biology· 0 citations
We use cookies to run the site and, with your consent, for analytics and to show ads.
See our Cookie Policy.
