Tongnao Decoction Facilitates Angiogenesis via the VEGFA/ PI3K/AKT/JUNB Signaling Axis to Promote Stroke Recovery.
Abstract
INTRODUCTION Acute ischemic stroke (AIS) is a leading cause of global disability and mortality, with treatments limited by narrow time windows. Tongnao Decoction (TND), a traditional Chinese medicine formula, has shown neuroprotective potential. This study investigates TND's mechanism in promoting post-stroke recovery.
Methods
Chemical analysis of TND using UHPLC-QTOF-MS identified 20 bioactive compounds. A photothrombosis-induced mouse stroke model was established and confirmed by TTC staining and MRI.
Results
Behavioral tests showed TND significantly improved motor function, an effect reduced by the VEGFR2 inhibitor SKLB1002.
Discussion
In human cerebral microvascular endothelial cells (HCMECs), TND (1375 μg/mL) enhanced viability by 53.3%, proliferation by 1.5-fold, migration by 73%, and tube formation by 1.3-fold. Western blot analysis revealed that TND upregulates p-PI3K/PI3K, p-AKT/AKT, and JUNB, which was reversed by SKLB1002.
Conclusion
In conclusion, TND facilitates functional recovery after AIS by promoting angiogenesis via the VEGFA/PI3K/AKT/JUNB pathway, supporting its therapeutic potential for ischemic stroke.