An integrated genome-wide resource reveals distinct replication environments of DNA breakage in human cancer cell lines

Replication stress in cancer: origins, consequences and therapeutic opportunities.

Genome-wide mapping of replication stress response factors reveals the management of polycistronic transcription, replication initiation, and responses to replication stress in Leishmania

Abstract Exposed single-stranded DNA (ssDNA) accumulating at stalled or collapsed replication forks (RFs) can trigger the replication stress response (RSR) to prevent genome instability. Leishmania are parasitic eukaryotes whose plastic genomes facilitate rapid adaptations under stress, with intrinsic replication stres...

Timing the origin: chromatin, transcription, and the spatiotemporal control of eukaryotic DNA replication

An updated framework for understanding replication timing as a key layer of genome regulation is provided, with emphasis on chromatin accessibility, histone modifications and variants, transcriptional regulators, and higher-order genome topology.

Single-cell mapping of chromosome breaks identifies multiple fragile site classes with distinct DNA replication timing landscapes

Common fragile sites (CFSs) are genomic regions susceptible to chromosome breaks. However, because they were identified using cell populations, low-frequency fragile sites (non-CFSs) could have been overlooked. Here we perform single-cell genome-wide mapping and classification of aphidicolin-induced chromosome breaks....

Replication stress at centromeres biases the segregation of DNA damage

Replication-associated errors can cause DNA damage to accumulate on the newly synthesized strand over time. In specific cases such as stem cells, retention of the “immortal” strand used as template preserves one daughter cell into pluripotency while correlating with terminal differentiation of the damage one. In somati...

Decoding single-cell replication states from imaging data reveals locus-specific subnuclear position dependencies of replication initiation