A structure-guided rational design to invert the coenzyme specificity of GDH by targeting a single residue within the conserved GXXXGXG motif of the Rossmann fold provides a generalizable framework for engineering cofactor preference in short-chain dehydrogenase/reductase family enzymes.
Vanillyl alcohol (VNA) is a high-value compound used in the pharmaceutical industry and other fields. Its microbial de novo biosynthesis is severely limited by the low catalytic efficiency of O-methyltransferase. Here, we constructed a V314I mutant of caffeate O-methyltransferase via structure-guided protein engineer...
Organofluorides are ubiquitous in modern society, but their persistence poses significant environmental and health risks. Fluoroacetate dehalogenases (FAcDs) are promising candidates for the bioremediation of polyfluorinated substances, as they can hydrolyze C–F bonds efficiently under mild conditions. However, their a...
Isobutene is an important platform chemical that is still predominantly produced from petroleum-derived feedstocks. The ferulic acid decarboxylase (Fdc) catalyzed decarboxylation of 3-methylcrotonic acid provides a green and sustainable route to bio-based isobutene. However, the poor activity of native Fdc toward this...
The direct asymmetric synthesis of chiral amides remains a focal point in green manufacturing, particularly for (S)-2-aminobutyramide whose traditional production is often hampered by low atom economy and arduous chiral resolution steps. Here, we report the creation of a non-native bio-amidation activity in Escherichia...
The structural, functional, and computational characterization of a chloramphenicol O-AT from Bacillus sp.
We use cookies to run the site and, with your consent, for analytics and to show ads. See our Cookie Policy.
