It is demonstrated that the Vibrio lateral flagellar filament is extensively modified by pseudaminic acid and that Maf is required for filament formation, the first structural characterization of a glycosylated Vibrio lateral flagellar filament and a close link between flagellin glycosylation and motility organelle assembly.
Salmonella can assemble functional flagella, even when the acetylglucosaminidase activity of FlgJ is genetically inactivated, showing that early steps in flagellar assembly are more diverse than previously thought and that differences in cell wall structure between gram-positive and -negative bacteria likely determine...
Yann H. U. Chevance, M. Kinoshita, F. Chevance et al.· Journal of Bacteriology· 0 citations
Flagella are large transenvelope nanomachines but how they transit the peptidoglycan in gram-positive bacteria is poorly understood. A recent model suggested that flagellar basal bodies diffuse in the membrane and become captured at locations in the peptidoglycan with a pore diameter that could accommodate the axle-lik...
Caroline M. Dunn, Kehinde O. Adebiyi, D. Kearns· Proceedings of the National...· 0 citations
Sortase A (SrtA) enzymes covalently anchor surface proteins to Gram-positive bacterial cell walls, promoting colonization and virulence. In Streptococcus pneumoniae, previous studies identified both a domain-swapped dimer and an active refolded monomer, but the active enzyme has not been characterized at the structural...
Eunjeong Lee, Blaine H. Gordon, J. Redzic et al.· Biomolecules· 0 citations
It is common for bacteriophages to encode proteins that are strongly inhibitory to growth of the bacterial host, and about 10% of mycobacteriophage-encoded proteins have this property. Adephagia is a Cluster K1 mycobacteriophage and prior cloning and expression of 66 Adephagia non-structural genes identified 14 that ar...
Michael J. Lauer, K. Freeman, Da Jiang et al.· bioRxiv· 0 citations
Non-tailed phages remain underexplored in marine environments, as tailed phages have long dominated sequence and culture collections. Yet recent surveys suggest that non-tailed phages may be more abundant and have distinct impacts on microbial mortality and gene transfer. Here, we solve the structure of Vibrio anguilla...
S. Otaegi-Ugartemendia, G. N. Condezo, Marta Martínez et al.· PLoS Pathogens· 0 citations
The bmsA gene of Pseudomonas alkylphenolica KL28T encodes a conserved protein involved in motility and multicellular biofilm formation. Sequence analysis revealed that BmsA contains an extracellular CHASE3 sensory domain, suggesting a role in environmental signal perception. To investigate its function, a CHASE3 domain...
Gangsu Ha, Meriem Harrache, Kyoung Lee· Letters in Applied Microbiol...· 0 citations
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