Detection and quantification of the 120 bp insert in the CYP51 upstream region of the wheat pathogen Zymoseptoria tritici associated with azole fungicide resistance using digital droplet PCR
Aug 2026· Journal of plant diseases and protection· Vol 133· 0 citations· 20 references
TL;DR
A ddPCR is developed to accurately detect the insert in individual isolates and to quantify in field populations and reveals how the Irish Z. tritici population has changed, with high variability in its frequency originally observed in the mid-2010 replaced by a relatively stable frequency at approximately 5% since from 2021 onwards.
Fusarium head blight (FHB) caused by Fusarium graminearum species complex (FGSC) is a devastating disease worldwide. The new succinate dehydrogenase inhibitor pydiflumetofen is highly effective against FHB, but its growing use raises resistance concerns. In this study, we identified four resistant Fusarium asiaticum is...
Yu-Di Ma, Qiao-Wan Chen, Gui-Ping Zhang et al.· Journal of Agricultural and...· 0 citations
This study provides the first report worldwide of SDHI resistance in Monilinia species field isolates linked to aa changes in the SDH subunits and combined SDHI and DMI resistance in peach orchards, highlighting the need for resistance monitoring and management.
J. Gelain, Johanna Wesche, E. Trotter et al.· Phytopathology· 0 citations
Results demonstrate a clear fungicide-driven species replacement, where the ecological success of C. fructicola is attributed to its dual resistance rather than physiological superiority, providing critical evidence of how long-term selection pressure reshapes pathogen population dynamics.
Abdulkareem Abdullahi, S. An, Soobin Park et al.· Plant Pathology Journal· 0 citations
Soft rot, caused by the phytopathogenic bacteria
Pectobacterium
and
Dickeya
, is a serious and destructive disease of potato worldwide. Symptoms caused by the two genera are indistinguishable. An effective diagnostic strategy is essential for controlling the disease and reducing losses in potato production. In th...
H. A. Tamur, Firas A. Ahmed· Frontiers in Horticulture· 0 citations
Botrytis cinerea, the causal agent of gray mold, is a major pathogen in strawberry production. Quinone outside inhibitor (QoI) fungicides have been widely used for disease control, but resistance driven by the G143A mutation in cytochrome b (cytb) has limited their field efficacy. This study evaluated the sensitivity o...
Hyeong-rok Jang, Hai-Feng Liu, Doeun Son et al.· Plant Pathology Journal· 0 citations
The discriminative conventional PCR assays targeting secY provide a rapid and accessible tool for subgroup differentiation and may facilitate large-scale epidemiological surveillance and future studies of host tolerance.
Rafael Toth, Nadine Fluck, Johannes Ritz et al.· Plants· 0 citations
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