Engineering Escherichia coli Nissle 1917 for the highly efficient biosynthesis of food-grade ergothioneine.

Bioengineering and physicochemical optimization of ergothioneine production by Aspergillus oryzae.

Ergothioneine (EGT) is a bioactive, rare variant of histidine with many applications in the medical, pharmaceutical, and food fields. Therefore, we aimed to investigate in this study the impact of genomic and physicochemical factors on EGT production by the industrial filamentous fungus Aspergillus oryzae. Firstly, to...

Efficient Production of Vanillyl Alcohol in Escherichia coli via Protein and Pathway Engineering

Vanillyl alcohol (VNA) is a high-value compound used in the pharmaceutical industry and other fields. Its microbial de novo biosynthesis is severely limited by the low catalytic efficiency of O-methyltransferase. Here, we constructed a V314I mutant of caffeate O-methyltransferase via structure-guided protein engineer...

Engineering the Metabolic Pathway of Escherichia coli for Efficient Biosynthesis of Lacto- N -tetraose

Lacto-N-tetraose (LNT), a core component of human milk oligosaccharides (HMOs), plays essential biological roles; however, its production remains challenging due to low conversion efficiency and the accumulation of intermediates. In this study, a systematic metabolic engineering strategy was applied to Escherichia co...

Menaquinone-7 production by engineered Saccharomyces cerevisiae through de novo Synthesis and fermentation optimization

Integrated Transporter Engineering and Semi-Rational Enzyme Design in Corynebacterium glutamicum for High-Level Natural Blue Pigment Indigoidine Production

Indigoidine is a sustainable natural blue pigment with broad industrial prospects, while its microbial production is hampered by growth inhibition of the producing strain, enzyme-host incompatibility, and insufficient industrial-scale titer. In this study, de novo biosynthesis of indigoidine was achieved in Corynebac...

High-level surfactin production by Bacillus subtilis through integrated metabolic engineering and bioprocess optimization.

The integration of multi-level strain engineering with fermentation optimization thus established B. subtilis ATCC 6051a as an effective platform for high-level surfactin production and provides a practical framework for the development of efficient microbial surfactin production systems.