Protein profiling and GC–MS product analysis provide insights into lignite solubilization and bioconversion by Lysinibacillus sphaericus strain SH19

Comparative proteomics and network analysis of the secretome of thermotolerant Ascomycetes for lignin degradation.

Lignin is one of the major bottlenecks for lignocellulosic biomass conversion into biofuels and value-added bioproducts by its recalcitrance. Isolates of (Aspergillus caespitosus) show very similar absolute ligninolytic activity, but the molecular basis for the superior ligninolytic performance of different isolates re...

Integrated Multi-Omics and Molecular Docking Analysis of Malate Degradation by Lactiplantibacillus plantarum S4 During Sea Buckthorn Juice Fermentation

Sea buckthorn juice (SBJ) is nutritionally valuable, but its high malic acid content contributes to excessive sourness that limits consumer acceptance. In this study, the deacidification phenotypes of two lactic acid bacterial isolates, Lactiplantibacillus plantarum S4 and Lacticaseibacillus paracasei YJ2, were compare...

Multiomics profiling of transcriptional and metabolic responses of Bacillus siamensis LSZ23 to corn straw

A multiomics framework for LSZ23-mediated lignocellulose bioconversion is provided and it is identified as a candidate hydrolytic partner for straw degrading consortia.

Enhancement of novel cello-xylosome saccharification performance via microbial surface display on lignocellulosic feedstocks.

Cellulosomes are regarded as the most intricate naturally occurring cellulolytic systems. This study reports the design and surface assembly of a novel artificial cello-xylosome on Escherichia coli BL21:ΔCsgA cells. This synthetic complex incorporates two highly active enzymes previously sourced from sheep rumen microb...

Characterization of a novel CBM-independent GH13_5 raw starch-degrading α-amylase from Priestia koreensis HL12 for maltooligosaccharide production

Ectoine Mitigates Stress and Enhances Lignin to Vanillin Bioconversion in Halalkalibacterium ligniniphilum L1.

Engineered microbial systems offer a route for converting lignin into value-added aromatics, yet lignin-derived intermediates are cytotoxic and suppress growth and metabolic flux. Here, we show that ectoine, a compatible solute, mitigates lignin-induced stress in Halalkalibacterium ligniniphilum L1 and improves bioconv...