Nucleic acid testing is important for infectious-disease diagnosis, but combining high sensitivity with accessible testing remains challenging. Here we show a chip-based visual detection strategy for one or two viral RNA targets that involves only sample loading, without further instrumentation or manual actuation. Target-activated CRISPR-Cas13a alters the wettability of a molecular barrier, causing liquid to cross a preset threshold and generate a dam-break drainage signal. The chip detects a single target at 10 aM within 2 min and two targets at 100 aM within 5 min, with costs of $0.20 and $0.30 per test, respectively. We further establish a practical clinical-sample workflow combining room-temperature lysis with RNase inhibition and validate the platform across 40 nasal-swab samples tested for SARS-CoV-2, influenza A virus and influenza B virus, with results concordant with RT–PCR. This threshold-based fluidic strategy provides a route towards rapid, low-cost and instrument-free molecular testing outside centralized laboratories.
Rapidly converting viral genome information into deployable molecular tests remains a major challenge in outbreak preparedness. We developed CONAN-SWIFT (Simple Workflow for Isothermal Field Testing), a sequence-to-test platform that integrates computational assay design, reverse-transcription loop-mediated isothermal...
Jun Nakamura, Kaya Miyazaki-Iida, Shotaro Torii et al.· bioRxiv· 0 citations
CATNAP (Cas trans -nuclease detection of amplified products), a method that integrates isothermal linear DNA amplification with Cas12a detection in a single reaction, bridges the sensitivity gap in CRISPR diagnostics while maintaining simplicity, making accurate disease detection more accessible in resource-limited setti...
Selma Sinan, Remy M. Kooistra, Karunya Rajaraman et al.· 0 citations
The design principles and working mechanisms of cascaded CRISPR strategies, encompassing Enzyme-Coupled cascades, Multi-effector class 2 CRISPR cascades, and Type III CRISPR-mediated cascades are introduced, including integration with digital detection platforms and AI-assistant algorithms are discussed.
Ruo-Nan He, Lu Miao, Rui-Jie Deng et al.· Biosensors & bioelectronics· 0 citations
Together, RTLC is successfully validated in practical samples by detecting lncRNA HULC and miR-21, offering a robust, versatile tool for high-performance nucleic acid diagnostics.
Rapid identification of bacterial pathogens remains challenging in clinical settings, particularly when timely decisions are required for acute or polymicrobial infections. Here, we report an amplification-free solid-state nanopore strategy for bacterial DNA detection using 12 nm silicon nitride nanopores and a minimal...
