Extracting and Quantifying Specific Pollen Proteins by Western Blot.

Quantitatively Analyzing the Structural Organization at the Tip of Growing Tobacco Pollen Tubes.

This chapter describes methods required for the generation of transgenic tobacco lines and for the expression level-independent quantitative characterization of PM domains and cytoplasmic regions at the pollen tube tip.

Evaluation of Pectin and Arabinogalactan Protein Distribution in Olive Pollen Tube Cell Walls Using Immunofluorescent Labeling.

Quantitative Profiling of Metabolites, Sugars, and Lipids in Pollen Tubes by Mass Spectrometry.

Protein Extraction Methods for Ustilago maydis.

Integrated Transcriptomic, Proteomic, and miRNA Profiling Reveals the Regulatory Mechanisms Underlying Pecan Seed Germination

This multiomics study provides insights into potential molecular regulatory processes underlying pecan seed germination and offers a theoretical basis for future functional studies, seedling cultivation, and breeding improvement.

Assessment of Pollen Viability and Germination Through Histochemical, In Vivo and In Vitro Methods.