AI Networking Cookbook: Practical recipes for AI-assisted network automation and development
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Pronounced cultivar × treatment interactions confirmed that PGPR efficacy is modulated by host genetic background, with resistant varieties carrying pyramided Xa resistance genes showing additive responses to biological treatment.
A functionally diverse seed endophytic community with complementary roles in plant growth and disease suppression is revealed and rational selection and combination of complementary strains, particularly N. cucumis WSSR17 (growth promotion), C. erzurumensis WSSR11 (biocontrol), and P. poae WSSR12 (multifunctional nutrient mobilization) could enable the development of targeted, multi-strain bioinoculant strategies for wheat.
BACKGROUND Fusarium crown rot (FCR), caused predominantly by Fusarium pseudograminearum, is a devastating soil-borne disease threatening global wheat production. Systematic discovery of keystone microbial taxa with biocontrol potential from the wheat microbiome remains poorly explored. This study aimed to identify core microbiome biomarkers associated with FCR resistance and functionally validate candidate biocontrol agents. RESULTS Bacterial and fungal communities across five wheat compartments (rhizosphere, root, stem, leaf, grain) were profiled under FCR challenge. Host compartment niche was the primary driver of wheat microbial community assembly. FCR infection reduced root and stem microbial α-diversity, strengthened homogeneous selection-dominated deterministic fungal assembly in stems, coincided with declined dispersal limitation in root bacterial assembly, and disrupted microbial network stability. Integrated analysis identified Bacillus ASV_2195, enriched in healthy wheat roots, as a core FCR resistance biomarker. The corresponding strain, Bacillus sp. XN303, was isolated. Whole-genome sequencing of XN303 uncovered gene clusters encoding antimicrobial compounds and plant-beneficial traits. Functionally, XN303 directly inhibited F. pseudograminearum growth by 71.64%, reduced the FCR disease index by 79.21%, and lowered pathogen density in rhizosphere soil and stems by 22.89% and 20.28%, respectively, in pot assays. In addition, XN303 demonstrated the capacity to detoxify deoxynivalenol (DON) and activate jasmonic acid-mediated defense priming in wheat. CONCLUSION Bacillus sp. XN303, identified through microbiome-guided screening, confers robust FCR protection via pathogen antagonism, DON detoxification, growth promotion, and defense priming, representing a potential candidate biocontrol agent for sustainable FCR management. © 2026 Society of Chemical Industry.
Results indicate that B. velezensis B05 has potential as a multifunctional biocontrol agent for sustainable management of soybean root rot.
BACKGROUND Modern agriculture urgently requires sustainable alternatives to synthetic chemical pesticides to mitigate crop yield losses while protecting human health and the environment. This study evaluated the biocontrol potential and plant growth-promoting (PGP) traits of three newly isolated Streptomyces strains (Strep_22, Strep_23, and Strep_24) against severe soilborne fungal pathogens affecting durum wheat (Triticum durum Desf.) and tomato (Solanum lycopersicum L.), two economically crucial crops. RESULTS In in vitro dual-culture assays, strain Strep_22, identified as Streptomyces netropsis, demonstrated the highest overall efficacy, achieving a mean fungal growth inhibition rate of 91.52% and near-complete suppression of critical pathogens such as Agroathelia rolfsii, Fusarium solani, and Sclerotinia sclerotiorum. Kinetic modelling confirmed that Strep_22 significantly reduced fungal growth rates. Based on these outstanding findings, Strep_22 was selected for in vivo glasshouse validation. Glasshouse trials demonstrated that treatments with this strain significantly reduced disease incidence and severity (such as root rot and wilt) in both wheat and tomato crops. Concurrently, Strep_22 exhibited strong PGP traits, inducing a significant increase in plant biomass, shoot height, and root development compared to untreated, infected controls. CONCLUSION The Streptomyces strain Strep_22 represents a highly promising, dual-action biocontrol agent. It effectively protects wheat and tomato crops against devastating fungal infections while simultaneously stimulating plant vegetative growth and development. Consequently, it constitutes a valuable ecological alternative for the formulation of commercial biofertilizers and biopesticides, paving the way toward sustainable agricultural systems. © 2026 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
The potential of P1 as a promising bioinoculant candidate for sustainable agriculture in the potato sector is demonstrated and the genome lacked major virulence factors and antimicrobial traits, supporting the non-pathogenic nature of the P1 strain.
Overall, Streptomyces virginiae JCK-8401 is a highly promising, multifunctional biocontrol agent for managing soil-borne diseases through a synergistic combination of antibiosis, bioinoculation, and the induction of host plant defense responses.
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