RNAi-based management of Ceutorhynchus obstrictus: opportunities, challenges, and research priorities

Abstract

RNA interference (RNAi) has emerged as a promising, highly specific and environmentally sustainable strategy for pest management. Given the urgent need for a targeted and effective solution against the cabbage seedpod weevil (CSW) Ceutorhynchus obstrictus (Marsham), a major pest of oilseed rape, RNAi-based management offers a promising opportunity. This review explores the application of RNAi within the Curculionidae family, with a focus on CSW, and evaluates the key challenges in implementation. A major barrier to effective RNAi in CSW is ensuring the stability of double-stranded RNA (dsRNA) until it reaches its target site. We examine the factors influencing dsRNA stability and their implications for RNAi efficacy. In addition, we discuss strategies for selecting suitable target genes and highlight potential candidate genes for silencing in CSW. Species-specific traits of CSW, including exoskeletal structure and feeding behavior, are also considered for their impact on dsRNA uptake and RNAi efficacy. While RNAi holds great potential, overcoming biological and technical barriers will be essential for optimizing RNAi-based control. This review provides insights into these challenges and outlines future directions for advancing RNAi-based pest management. By addressing current limitations and improving methodologies, RNAi can become a promising and sustainable method for controlling CSW populations.