Bacterioruberin is a C50 carotenoid gaining more interest because of its important biological properties. This study aimed to the identification and characterization of a bacterial strain producing this attracting carotenoid, Arthrobacter sp. strain CP30. Phylogenetic analysis based on 16S rRNA gene sequences and whole genome comparison showed that strain CP30 belongs to the Arthrobacter agilis group with Arthrobacter bussei being the closest relative. Sequence similarity of the 16S rRNA gene of CP30 to described Arthrobacter species as well as average nucleotide identity values indicate that CP30 represents a new species within the genus Arthrobacter. The strain has been cultivated under controlled fermentation parameters, and the obtained biomass was investigated for carotenoids and lipids quali-quantitative profiles characterization. The biomass extracts enriched in bacterioruberin have been evaluated for antimicrobial and antioxidant properties. The bacterioruberin produced by the strain reached up a carotenoid titer of 1.28 mg L-1 in 72 h, with a yield of 327.67 ± 6.6 μg g-1 of dry biomass, a productivity of 0.02 mg/L/h, and showed an IC50 value of 3.87 μg/mL. The most abundant fatty acids were 12-methyltetradecanoic, 14-methylpentadecanoic and 13-methyltetradecanoic. Whereas, among the intact lipids, glycolipids, phospholipids, diacylglycerols, and triacylglycerols have been identified in the investigated strain. This study allowed pointing out the potential of strain CP30 as a promising bacterioruberin producer, representing an interesting natural carotenoids source to be further investigated as suitable candidate for feed and food application. Comparison of key protein sequences involved in bacterioruberin biosynthesis of CP30 and A. bussei showed sequence similarities of >90%.
The rise of antimicrobial resistance and emerging infectious diseases necessitates the discovery of novel antibacterial agents. Endophytic actinomycetes, especially Streptomyces species colonizing medicinal plant tissues, constitute a reservoir of structurally diverse bioactive metabolites that remains insufficiently characterized. This study reports the isolation and comprehensive characterization of an endophytic actinomycete, designated strain YBRX0, recovered from the root tissues of the Vietnamese medicinal plant Croton tonkinensis Gagnep. Strain identity was established through an integrated approach combining colony morphology, scanning electron microscopy, physiological and biochemical profiling, and 16S rRNA gene phylogenetic analysis. Bioactivity screening of the ethyl acetate fermentation extract employed agar well diffusion to assess antibacterial spectrum, with inhibitory potency quantified by broth microdilution. Antioxidant capacity was measured via DPPH radical scavenging, and secondary metabolite composition was profiled by GC–MS. The integrated characterization placed strain YBRX0 unambiguously within the genus. At 1 mg/mL, the ethyl acetate extract demonstrated broad-spectrum activity against both Gram-positive and Gram-negative pathogens, with MIC values ranging from 8 to 16 μg/mL. Antioxidant screening yielded an IC₅₀ of 197.26 ± 8.87 μg/mL. GC–MS tentatively identified Pyrrolopyrazine diketopiperazines and Benzeneacetamide as principal constituents. Collectively, these findings indicate that the crude extract of Streptomyces sp. YBRX0 exhibited notable antibacterial activity despite being an unfractionated mixture, supporting its potential as a promising source of bioactive metabolites for further bioprospecting and compound isolation efforts targeting drug-resistant pathogens.
Hà Trang Phan, Hung Vuong Pham, Xuan Truong Nguyen et al.· Journal of Genetic Engineeri...· 0 citations
Overall, UzMU25 exhibited preliminary enzymatic, antagonistic, and biofilm-forming characteristics of biotechnological interest, but whole-genome antimicrobial-resistance screening and more comprehensive phenotypic and toxicological evaluations are required before the strain can be recommended for probiotic or other practical applications.
Luiza Tagayeva, K. Normurodova, S. Jabborov et al.· Microorganisms· 0 citations
Exopolysaccharides (EPS) are microbial biopolymers composed primarily of complex carbohydrates and are valued for their biocompatibility, biodegradability, and diverse biological activities. In this study, lactic acid bacteria were isolated from sugarcane waste, and a potent EPS-producing strain was identified as Weissella paramesenteroides through 16S rRNA gene sequencing. The sequence was deposited in GenBank under accession number PV786832. EPS production was optimized using a One-Factor-At-a-Time (OFAT) approach. Maximum EPS yield was obtained at pH 7, 30 °C, and 48 h incubation, with sucrose and yeast extract serving as the most effective carbon and nitrogen sources, respectively. Chemical analysis revealed a total carbohydrate content of 90% and a residual protein content of 3.7%. Structural characterization confirmed the presence of characteristic polysaccharide functional groups in the EPS. The EPS exhibited antibacterial activity against both Gram-positive and Gram-negative pathogens and demonstrated antioxidant properties, with a DPPH IC50 value of 188.6 µg/mL, ferric reducing antioxidant activity of 51.47 µg Trolox equivalents, and total antioxidant capacity of 160.02 ± 2.91 µg AAE/mL extract. Furthermore, the EPS showed dose-dependent anti-inflammatory activity with an IC50 value of 403.9 µg/mL. These findings highlight the bioactive potential of EPS from W. paramesenteroides and support its further exploration for biotechnological applications.
Among the 20 isolates, one isolate was identified as Brevibacillus parabrevis PS12, representing the first report from Iraqi soil, which contributed to an increase in bacterial biomass which led to a rise in the specific activity of arginase.
Ayah Muwafaq Hussein, Aqeel Mohammed Majeed Al-Ezee, Zaid Raad Abbas· Journal of the College of B...· 0 citations
Among 14 endophytic isolates, Bacillus subtilis LS1-5 showed the strongest antifungal activity by dual culture assay, and exhibited a broad array of plant growth-promoting and environmental resilience traits.
D. Nguyen, Dong Tang Vien, Ngoc-Minh Truong Bui et al.· IOP Conference Series: Earth...· 0 citations
The integrated experimental and in silico approach highlights soil-derived actinomycetes as versatile and sustainable bioresources with significant pharmaceutical and biotechnological potential, emphasizing their role in combating antimicrobial resistance and enabling the rational development of novel therapeutic and industrial products.
Meghana Arivilu, Shaziya Sulthana, Vijay Ramesh et al.· Journal of Pure and Applied...· 0 citations
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