A high-quality chromosome-level reference genome assembly of Triosteum pinnatifidum (Caprifoliaceae)
TL;DR
A high-quality chromosome-level genome assembly of Triosteum pinnatifidum is reported using PacBio HiFi long-read sequencing and Hi-C scaffolding technologies, indicating a high level of assembly accuracy and completeness.
Abstract
Triosteum pinnatifidum is a perennial herbaceous plant endemic to East Asia, whose roots, leaves, and fruits have long been utilized in traditional herbal medicine, thereby demonstrating substantial medicinal value. The root of T. pinnatifidum (commonly referred to as “Tianwangqi”) exhibits multiple pharmacological effects: enhancing Qi-blood circulation, dispelling wind-dampness, tonifying the spleen and stomach, and relieving inflammation and pain. However, the absence of a reference genome has impeded genetic research on this species. In the present study, we report a high-quality chromosome-level genome assembly of T. pinnatifidum generated using PacBio HiFi long-read sequencing and Hi-C scaffolding technologies. The final assembled genome spans 698.94 Mbp with a contig N50 of 46.55 Mbp, which was successfully anchored onto 9 chromosomes. Genome annotation revealed that repetitive sequences account for 65.84% of the genome, and a total of 26,027 protein-coding genes were predicted, among which 98.19% were functionally annotated. The final gene set of T. pinnatifidum exhibited a BUSCO completeness of 98.5%, indicating a high level of assembly accuracy and completeness. This genome assembly not only contributes to the genetic conservation of Triosteum L. species but also provides a valuable genomic resource for evolutionary studies within the Caprifoliaceae family.