Ansacarbamitocin B and D isolated from Amycolatopsis sp. FID23-7-18 associated to the fungus-growing ant Mycetomoellerius mikromelanos.

Abstract

Ansacarbamitocin B (1) and D (2) were identified as major compounds from ISP-2 agar culture of Amycolatopsis sp. FID23-7-18, isolated from the exoskeleton of Mycetomoellerius mikromelanos worker ants. Elucidation of their structures was accomplished using 1D and 2D NMR spectroscopy and MS data. Compounds 1 and 2 showed antifungal activity against the mycoparasite Escovopsis sp. and inhibited the growth of MCF-7, SF-268, and NCI-H460 cancer cell lines with an IC50 of 0.42 to 12.0 µM. Both compounds showed no evident activity against bacteria (Staphylococcus aureus ATCC 25923, Escherichia coli CETC 434, Enterococcus faecalis ATCC 19433, and Pseudomonas aeruginosa ATCC 10145), yeast-like fungus (Candida albicans ATCC10231), and protozoa (Leishmania donovani, Plasmodium falciparum, and Trypanosoma cruzi).