Evaluation of Forensic Genetic Efficiency Parameters of 23 Autosomal STR Markers (PowerPlex® Fusion 6C system) in a Population Sample of Hadoti Region

Abstract

Rajasthan’s southeastern part is known as “the land of Hada (Clan) Rajputs” i.e Hadoti. In this study, genetic structure of Hadoti population, who exhibited diverse ethanographic past, have been explored using 23 autosomal STR loci [PowerPlex® Fusion 6C (PPF) system] by the way of determining allele distribution and forensic ef𿿿ciency parameters. Autosomal STR data was collected fromthe bloodsamples of 423 (194 females and 229 males) individuals of Rajasthani roots related to the Hadoti region. The STRs were ampli𿿿ed by using PPF 6Csystem(PromegaCorporation) and the ampli𿿿ed samples were examined using Gene Mapper ID-X 1.6 software (Applied Biosystems) on a 3500xl Genetic Analyzer. Following Bonferroni correction, all the studied STR loci ful𿿿lled the terms of the HardyWeinberg equilibrium. With the exception of Amelogenin and DYS391, DYS570, DYS576, this study found that among the 23 autosomal STR loci, SE 33 is the most informative (power of discrimination (PD) = 0.992), whereas TPOX locus (PD = 0.866)is the least. The obtained parameters of forensic interest demonstrated the compatibility of all the studied 23 STRs across Hadoti population of Rajasthan for forensic DNA identi𿿿cation and paternity testing.