Expression, Purification, and Structural Characterization of Membrane Proteins: A Case Study of the ABC Transporter MsbA.

Expression and Purification of Integral Membrane Enzymes for Functional and Structural Studies.

Together, the methods described here demonstrate that careful selection via thorough screening, and optimization of expression and purification conditions, are an invaluable investment of time, ultimately yielding the best target not only for successful high-resolution structural analyses-X-ray crystallography and cryo...

A generalisable method for the purification and biophysical characterisation of bacterial membrane receptors

A generalizable workflow for producing soluble receptor domains from membrane proteins is established and validated HasR constructs suitable for downstream ligand-screening applications, including aptamer and nanobody discovery.

Purification and Sonication-Assisted Cryo-EM Grid Preparation of TolC.

Expression and Purification of a Multidrug Transporter PfMATE for Structural Analyses Using Solution NMR.

Isolation of AtSWEET Membrane Proteins from Arabidopsis Leaves for Host-Pathogen Interaction Studies.

The current protocol outlines a method for membrane purification utilizing ultracentrifugation and detergents, which facilitates the isolation of membrane proteins in their native conformational state.

Structural modelling and functional analysis support lipid binding by the dimeric E. coli DedA protein YqjA

It is shown that the Escherichia coli DedA protein YqjA exists predominantly as a dimer and, by combining structural modelling with site-specific cysteine crosslinking, the most likely dimer interface is identified, which supports a role for lipid binding in the activity of YqjA.