2026· Methods in molecular biology· Vol 3047, pp.
127-139
· 0 citations
Medicine
TL;DR
This work presents a robust RNA FISH protocol for detecting the lncRNA Xist, a chromatin-associated master regulator of X chromosome inactivation (XCI) that serves as a paradigm for lncRNA biology and demonstrates the effectiveness of this method for investigating lncRNA localization and chromatin-associated function.
This chapter presents RNA Antisense Purification-RNA coupled to psoralen crosslinking (RAP-RNA[AMT]), a targeted method to capture direct RNA-RNA base-paired partners of a chosen lncRNA in its native cellular context.
Sylvia L. R. Wood, Bridget Flowers, Jong-Sun Lee· Methods in molecular biology· 0 citations
This chapter explores the biomedical significance of ceRNAs, highlighting their role in modulating gene expression and their potential implications in disease, and summarizes available tools, resources, and practical examples for studying lncRNA-miRNA interactions to aid researchers in navigating this sophisticated reg...
Francesco Orilio, R. Brancaccio, Francesco Verona et al.· Methods in molecular biology· 0 citations
Each isoform was assigned a cytoplasmic–nuclear relative concentration index (CN-RCI) measured from ENCODE subcellular fractionation RNA-seq across 15 human cell lines, and masked genes with both tissue-dominant isoforms localized were identified.
Hidenori Tani· Zeitschrift für Induktive Ab...· 0 citations
