Efficient Screening of High Bacterial Cell-Selective Antimicrobial Peptides via Proline Regulation Combined with a Universal Secondary Structure Template.
To study the ″preference″ of bacterial membranes for the secondary structure of antimicrobial peptides (AMPs), we designed a universal palindromic sequence template G(LK)n(KL)nG-NH2 (designated as GnPm, where n = 2-6 and m = 0-3) that can cover various secondary structures of AMPs, with 0 to 3 proline residues uniforml...