Allium aktauense
is a narrow endemic species from the Aktau Range in the Nurotau Mountains, Western Pamir-Alay, where it grows in rocky limestone crevices. Genomic resources for this species have not previously been available, limiting molecular understanding of its phylogenetic placement and conservation value. In this study, we sequenced, assembled and characterized the complete chloroplast genome of
A. aktauense
and evaluated its phylogenetic position within
Allium
using comparative plastome data. The plastome exhibited the typical circular quadripartite structure of angiosperms, with a total length of 152,443 bp, including a large single-copy region of 81,522 bp, a small single-copy region of 17,907 bp and two inverted repeat regions of 26,507 bp each. The overall genome organization, gene order and plastome structure were conserved, with no major rearrangements detected. The highest nucleotide diversity was observed in the
ndhF–rpl32
region, suggesting its potential utility as a molecular marker for future studies. Phylogenetic analysis based on complete chloroplast genome sequences placed
A. aktauense
within subgenus
Allium
, close to
A. ophiophyllum
, with
A. nikolaii
and
A. filidens
forming a neighbouring lineage. These results provide the first plastome resource for
A. aktauense
and contribute to future phylogenetic, taxonomic and conservation-related studies of endemic
Allium
species in Central Asia.
I. Ergashov, Farkhodjon Mingboev, Ziyoviddin Yusupov et al.· F1000Research· 0 citations
Poa
L. (Poaceae), comprising over 500 species, is taxonomically challenging due to morphological plasticity, hybridization, and polyploidy. To clarify plastome architecture and phylogenetic relationships within the genus, we sequenced and annotated complete chloroplast genomes of
Poa angustifolia
(accessions P10, P14) and
Poa lipskyi
(P17) from Uzbekistan. The three plastomes (135,310–135,609 bp) shared the typical quadripartite structure and an identical complement of 130 genes across self-replication, photosynthesis, and other functional categories. Codon usage and amino acid composition were highly conserved, with a consistent bias toward A/T-ending codons and leucine, isoleucine, glycine, and serine as the dominant residues. Sliding-window analysis identified two nucleotide-diversity hotspots, in the
rpl32
–
trnL
-UAG and
trnC
-GCA–
rpoB
regions, and SSR profiling showed predominant mononucleotide A/T repeats alongside a few taxon-specific pentanucleotide motifs. Maximum-likelihood phylogenomic analysis placed the
P. angustifolia
accessions in a strongly supported clade sister to
P. lipskyi
, consistent with shared membership in sect.
Poa.
These results indicate that
Poa
plastomes are structurally conserved yet retain informative variable regions useful for phylogenetics and barcoding.
Sh. V. Abdullaev, Temur Asatulloyev, Ziyoviddin Yusupov et al.· F1000Research· 0 citations
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