Rational design and molecular docking-guided screening of high-affinity β-glucosidase affibodies for application in a lateral flow immunoassay platform.
Intestinal microbial β-glucosidase serves as a key functional biomarker associated with gut dysbiosis and colorectal cancer (CRC) progression. However, the development of rapid and sensitive point-of-care testing (POCT) methods for β-glucosidase has been limited by the lack of stable and high-affinity recognition eleme...