A Time-Gated FRET Platform for Screening SARS-CoV-2 −1 Programmed Ribosomal Frameshifting Inhibitors
To address the emerging need for high-throughput and sensitive methodologies in RNA-targeted drug discovery, this study developed and validated a novel biosensing platform based on time-gated Förster resonance energy transfer (TG-FRET). The highly conserved RNA pseudoknot of SARS-CoV-2 served as our model system and...