We develop a marginal coordinate test for regression with Euclidean predictors and a random-object response in a separable metric space. The goal is to test whether a predictor provides additional information about the response conditional on the remaining predictors. In a semi-supervised design, an unlabeled sample is used to estimate predictor conditional means, while an independent labeled sample is reserved for inference. The resulting residuals are combined with a product-space kernel to form a kernel conditional mean dependence (KCMD) U-statistic without requiring a response residual. The primary identity-based test targets a necessary conditional mean restriction, while a multiple-transformation extension probes broader alternatives. We establish a weighted centered chi-square null limit, wild bootstrap validity, consistency against fixed detectable alternatives, and local power under mean-element alternatives. For simultaneous inference, truncated p-to-e calibration combined with e-BH provides asymptotic false discovery rate control under general dependence. Simulations with Euclidean and non-Euclidean responses, together with a New York City taxi-flow analysis, illustrate the method.
Jia-Ye Chen, Rui Qiu, Roulin Wang et al.· 0 citations
The rapid advancement of spatial transcriptomics has substantially improved our understanding of the spatial architecture and gene expression heterogeneity within tissues. However, many spatial transcriptomics techniques can not reach single-cell resolution, instead measuring gene expression profiles from mixtures of potentially heterogeneous cell types. Here we propose AddaGCN, a robust deconvolution method to infer cell type composition from spatial transcriptomic data. AddaGCN leverages graph convolutional networks to incorporate spatial information and adopts an adversarial discriminative domain adaptation approach to mitigate batch effects between spatial and single-cell reference data. Comprehensive analyses of real data generated by diverse technology platforms demonstrate AddaGCN’s superior performance and robustness in cell-type deconvolution compared to other methods. These analyses further reveal AddaGCN’s potential to uncover spatiotemporal changes during tissue development and to characterize the tumor microenvironment.