Zhi-Qiang Liu

Fine-Tuning l -Arginine Biosynthesis and Bidirectional Dynamic Control Overcome Metabolic Bottlenecks for High-Titer l -Ornithine Production in Escherichia coli

l-Ornithine is a versatile amino acid with broad applications in the pharmaceutical, nutraceutical, and food industries. However, its efficient microbial production is constrained by a critical trade-off: excessive blockage of the downstream l-arginine pathway severely impairs cell growth, while intracellular l-ornit...

Key enzyme optimization and multi-node metabolic flux regulation drive l-arginine production in Escherichia coli.

Microbial production of l-arginine is often constrained by tight metabolic regulation and insufficient precursor supply. Here a plasmid-free, non-auxotrophic Escherichia coli strain for high-level production of l-arginine was rationally engineered, based on our previous constructed strain G0 with 12.4 g/L l-arginine pr...

[Machine learning-based optimization strategies for fermentation processes].

This paper systematically reviewed the latest research progress on the closed-loop intelligent fermentation control systems, spanning from underlying data perception to high-level decision-making and the transformation of fermentation control from traditional "macroscopic feeding regulation" to "microscopic metabolic p...

Enhanced d -Pantothenic Acid Biosynthesis in Corynebacterium glutamicum by the CPSPC System and Spatial Organization Strategy

d-Pantothenic acid (D-PA) is an essential precursor of coenzyme A and the acyl carrier protein. Its biosynthesis requires abundant precursors and precise regulation of the multifunctional enzyme, ketopantoate reductase. Here, we engineered Corynebacterium glutamicum for enhanced D-PA production through metabolic engi...

Combinatorial Metabolic and Process Engineering for Enhanced GA4+7 Production and Tunable GA4/GA7 Ratio in Fusarium fujikuroi.

This study demonstrated that the GA4/GA7 ratio can be modulated through both molecular and fermentation strategies, and engineering a high-yielding GA4+7 producer using this Δp450-3 mutant as a platform.

Reconstruction and metabolic regulation of O-succinyl-L-homoserine biosynthesis pathway in Escherichia coli W3110.

The plasmid-free system constructed in this study effectively avoids the plasmid-induced metabolic burden and genetic instability and demonstrates the prominent advantages and great application potential of plasmid-free modular engineering for the efficient biosynthesis of OSH and other high-value amino acids.

Enhancing d-Pantothenic Acid Production in Corynebacterium glutamicum via Integrating Dynamic Regulation With Structure-Guided Protein Engineering of Ketopantoate Reductase.

This study establishes a robust chassis for sustainable DPA production and provides a generalizable framework for engineering other valuable biochemicals.

Enhanced Adenosine Biosynthesis in Paecilomyces hepiali Through Strain Evolution and Transcriptomic Insights.

An integrated strategy combining protoplast-based strain evolution, medium optimization, and transcriptomic analysis to enhance adenosine biosynthesis in P. hepiali is developed and transcriptomic insights into adenosine biosynthesis are provided and a practical framework for improving fungal nucleoside cell factories...

Transaminases engineering and their cascade systems for natural product synthesis.

Beyond direct pathway engineering: reprogramming Fusarium fujikuroi from a GA3 producer into a GA4+7 factory.

This study achieves indirect metabolic reprogramming not via conventional pathway engineering, but by targeting a membrane transport bottleneck and employing a putative post-transcriptional silencing mechanism.

Active Pocket Engineering of d-Tagatose 4-Epimerase for Improved Catalytic Performance and Efficient Cascade Synthesis of d-Tagatose from d-Glucose.

d-Tagatose is a rare hexose sugar with excellent properties, and its synthesis catalyzed by d-tagatose 4-epimerase (T4E) represents a competitive novel pathway. In this study, EbT4E derived from the Eubacteriales bacterium was screened and systematically characterized. By reshaping the microenvironment of the active po...