Zheng-Yi Zhang

Engineering of Redox-Active Residues Enhances P450 Peroxygenase Performance.

Cytochrome P450 peroxygenases depend on the high-valent iron-oxo intermediate Compound I (Cpd I) to activate C-H bonds. However, the nonproductive oxidative damage caused by Cpd I compromises enzyme stability and limits catalytic efficiency. Here, we report a rational protein engineering strategy to address this bottle...