Screening a thermotolerant Komagataella phaffii strain with methanol cross-tolerance and the associated physiological and transcriptional responses
Komagataella phaffii is a prominent methylotrophic yeast widely used in industrial biotechnology for heterologous protein production, chemical synthesis, and C1 feedstock utilization. However, its industrial application is limited by thermal stress and methanol toxicity, while the relationship between heat adaptation a...