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Continuous Evolution System Based on Toxin–Antitoxin System Combined with Base Deaminase Accelerates the In Vivo Modification of Target Proteins
The results demonstrate that PEACE is an efficient, versatile, and robust platform for in vivo continuous evolution with broad potential in protein engineering.
Rational engineering of cutinase for enhanced biodegradation of polyvinyl acetate and polyacrylates to control stickies in pulp and paper processing.
In the pulp and paper industry, residual sticky substances accumulate during white water circulation, forming deposits that cause paper defects and increase costs. Cutinase is a promising biocatalyst for mitigating stickies, but its industrial application is limited by inherent catalytic constraints. To address this, a...