Engineering yeast surface display of cutinase from Paraphoma chrysanthemicola for polyester plastic biodegradation.
A yeast surface display system in Pichia pastoris GS115 was developed for cutinase PcCUT7 obtained from Paraphoma chrysanthemicola. GCW61, a glycosylphosphatidylinositol-modified cell wall protein, was used as the anchoring module. PcCUT7 was genetically fused to GCW61 and successfully displayed on the yeast cell surfa...