Synergistic cascade amplification by catalytic hairpin assembly and terminal deoxynucleotidyl transferase for ultrasensitive detection of microRNA-21.
A highly sensitive electrochemical biosensor, using ordered mesoporous carbon (OMC-COOH) as the electrode substrate material, combined with the catalytic hairpin assembly (CHA) reaction and the cascade amplification effect of terminal deoxynucleotidyl transferase (TdT) to achieve quantitative detection of miRNA-21.