Bisulfite (HSO3-) is widely used as a food additive, yet its excessive residue poses potential health risks. Herein, a novel fluorescent probe TC-FV-BT was developed for the detection of HSO3-. Upon addition of HSO3-, the nucleophilic addition disrupted the π-conjugation of the probe, giving rise to a distinct fluorescence enhancement with bright orange emission. TC-FV-BT exhibited a rapid response, a low detection limit of 0.077 μM, and excellent selectivity. The sensing mechanism was confirmed by 1H NMR titration, HRMS, and DFT calculations. Furthermore, TC-FV-BT was successfully immobilized onto paper, cellulose acetate (CA), and chitosan (CS) film substrates to construct a portable visual detection platform, which was applied to the analysis of HSO3- in ten complex food matrices. Notably, the CS film-based sensor displayed superior anti-interference performance in high-oil and high-pigment samples. This work provides a versatile and practical platform for on-site rapid monitoring of HSO3- in food safety applications.
Herein, we have developed a novel ratiometric fluorescent probe through rational design and synthesis, named 2-((5-(4-(3,3-dimethyl-1,2,3,4-tetrahydro-2,4-methoxyacridin-9-yl) phenyl) thiophen-2-yl) methylene)-1H-indene-1,3 (2H)-dione, abbreviated as TA-PT-ID. TA-PT-ID is based on the nopinone skeleton and utilizes a Michael addition reaction as the GSH recognition mechanism. TA-PT-ID exhibits excellent photophysical properties, including a rapid response to GSH (within 1 min), a good linear correlation (R2 = 0.9919), and a low detection limit of 50 nM. TA-PT-ID was successfully applied to the detection of GSH in soil and water samples. Furthermore, ratiometric imaging of endogenous GSH was achieved using TA-PT-ID in HeLa cells, RAW264.7 cells, and zebrafish. In summary, TA-PT-ID provides a powerful new tool for the real-time detection of GSH in complex environments, and its rapid response and broad pH adaptability make it highly promising for applications in biological imaging and environmental monitoring.
Mengdi Zhao, Yue Gu, Yueyin Liang et al.· Spectrochimica Acta Part A -...· 0 citations
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