Activity-guided native-PAGE electroelution coupled with DIA proteomics uncovers the superoxide dismutase and antioxidant network in mature peanut seeds.
An activity-guided workflow that combines native-PAGE NBT zymography, low-temperature dialysis-bag electroelution and DIA-based nano-LC-MS/MS to enrich native SOD complexes from seed crude extracts resolves the hidden antioxidant proteome of peanut seeds, providing a general strategy for studying isoenzyme repertoires and redox protein networks in crop seeds.