AI Networking Cookbook: Practical recipes for AI-assisted network automation and development
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This work establishes a modular platform for efficient production of vanillin and its derivatives in E. coli by enhancing SAM biosynthesis, accelerating S-adenosyl-l-homocysteine (SAH) hydrolysis, and establishing methionine regeneration.
Vanillyl alcohol (VNA) is a high-value compound used in the pharmaceutical industry and other fields. Its microbial de novo biosynthesis is severely limited by the low catalytic efficiency of O-methyltransferase. Here, we constructed a V314I mutant of caffeate O-methyltransferase via structure-guided protein engineerin...
Daphnetin is a clinically established coumarin, but its native biosynthetic pathway remains elusive. In this study, a 2-oxoglutarate-dependent dioxygenase (2OGD), AtS8H, was identified that hydroxylates umbelliferone (UMB) to daphnetin , enabling the design of an artificial biosynthetic pathway. However, the pathway ef...
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