Aug 2026
Programmable Allosteric DNAzyme Coupled with CRISPR/Cas12a System for Multiplexed and Sensitive Detection of Extracellular Vesicle Derived MicroRNAs
A previously unrecognized feature of CRISPR/Cas12a is identified, in which incorporation of ribonucleotides into single stranded DNA targets modulates Cas12a activation efficiency, revealing a hybrid DNA/RNA-dependent regulation of Cas12a activity.
Xiang-Lan He, Le Wang, Cong Zhang et al.
· bioRxiv · 0 citations