Development and validation of a multiplex TaqMan real-time quantitative PCR assay for the simultaneous detection of six major bacterial respiratory pathogens in goats
Background Respiratory disease caused by bacterial and mycoplasmal pathogens is an important threat to goat health, and mixed infections complicate etiological diagnosis. Rapid multiplex detection is therefore valuable for clinical investigation and surveillance. Methods Two triplex TaqMan real-time quantitative PCR (q...